Excretion of matrix metalloproteinases 2 and 9 in urine is associated with a high stage and grade of bladder carcinoma
Excretion of matrix metalloproteinases 2 and 9 in urine is associated with a high stage and grade of bladder carcinoma
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DOI：
10.1016/s0090-4295(00)01087-6
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发表时间：
2001-04-01
期刊：
影响因子：
2.1
通讯作者：
Loening, SA
中科院分区：
文献类型：
--
作者：
Gerhards, S;Jung, K;Loening, SA
目标。目的分析膀胱癌患者尿液中基质金属蛋白酶（MMPs） 2和9的分泌情况，并根据肿瘤分期和分级评价其诊断的临床有效性。在许多癌症中，MMPs的表达增加与恶性程度较高和预后不良有关。研究人群包括44名无恶性肿瘤证据的对照组、14名膀胱炎患者、43名Ta-T1期患者、18名T2期患者和10名T3-T4期膀胱癌患者。用明胶酶谱法测定尿样品中MMP-2和MMP-9的排泄量，并与尿肌酐浓度相关。资料评价采用单变量统计分析、logistic回归分析和受试者工作特征分析。结果：MMP-2和MMP-9排泄上限分别为277杯/g肌酐和648杯/g肌酐。MMP-2、MMP-9水平与肿瘤分期、分级相关。排泄增高主要见于侵袭性肿瘤患者（T2-T4期）。在受试者操作特征分析中，肌肉侵袭性肿瘤患者的MMP-2和MMP-9曲线下面积明显高于非侵袭性肿瘤患者。与临界值相关，MMP-2检测膀胱癌的总灵敏度为0.51，MMP-9为0.31。logistic回归分析中，MMP-2表现最佳。尿中MMP-2和MMP-9的分泌与膀胱癌的高分期和分级相关，它们可能是未来肿瘤进展和复发的指标。中华泌尿外科杂志（英文版），2001。(C) 2001, Elsevier Science Inc.；
Objectives. To analyze the excretion of matrix metalloproteinases (MMPs) 2 and 9 in the urine of patients with bladder cancer according to the stage and grade of tumor and to evaluate their diagnostic clinical validity. In numerous carcinomas, increased expression of MMPs is associated with a higher grade of malignancy and poor prognosis.Methods. The study population included 44 controls without evidence of malignancy, 14 patients with cystitis, and 43 patients with Stage Ta-T1, 18 patients with Stage T2, and 10 patients with Stage T3-T4 bladder cancer. MMP-2 and MMP-9 excretion in urine samples was measured with gelatin zymography and related to the urine creatinine concentration. The evaluation of data was performed by univariate statistical analysis, logistic regression analysis, and receiver operating characteristic analysis.Results, The upper cutoff limit for MMP-2 and MMP-9 excretion was 277 mug/g creatinine and 648 mug/g creatinine, respectively. Levels of MMP-2 and MMP-9 correlated with each other and with tumor stage and grade. Elevated excretions were mainly observed in patients with invasive tumors (Stage T2-T4). In the receiver operating characteristic analysis, the areas under the curves for MMP-2 and MMP-9 were significantly higher in patients with muscle-invasive than in patients with noninvasive tumors. Related to the cutoff limits, the overall sensitivity to detect bladder cancer was 0.51 for MMP-2 and 0.31 for MMP-9. In logistic regression analysis, MMP-2 showed the best results.Conclusions. Urinary excretion of MMP-2 and MMP-9 is associated with a high stage and grade of bladder cancer, and they may serve as indicators of tumor progression and recurrence in the future. UROLOGY 57: 675-679, 2001. (C) 2001, Elsevier Science Inc.