Rabbit lung flavin-containing monooxygenase is immunochemically and catalytically distinct from the liver enzyme.
Rabbit lung flavin-containing monooxygenase is immunochemically and catalytically distinct from the liver enzyme.
复制标题
兔肺含黄素单加氧酶在免疫化学和催化方面与肝酶不同。
DOI：
10.1016/s0006-291x(84)80342-3
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发表时间：
1984
影响因子：
3.1
通讯作者：
Masters,BS
中科院分区：
文献类型：
--
作者：
Williams,DE;Ziegler,DM;Nordin,DJ;Hale,SE;Masters,BS
从孕兔肺微粒体中纯化了含黄素的单加氧酶。在豚鼠体内产生抗兔肺黄素单加氧酶抗体。该抗体的Ouchterlony双扩散分析产生了与纯化的兔肺酶和妊娠和非妊娠兔肺微粒体相同的沉淀素线。与兔肝微粒体或纯化的猪肝酶无交叉反应，三环类抗抑郁药丙咪嗪和氯丙嗪不是兔肺酶的底物，而它们被猪肝酶迅速氧化，这些结果表明兔肺和肝含黄素单加氧酶的底物特异性不同，是免疫化学上不同的蛋白质，可能是不同的基因产物。
Flavin-containing monooxygenase has been purified to homogeneity from lung microsomes of pregnant rabbits. Antibody to rabbit lung flavin-containing monooxygenase was raised in guinea pig. Ouchterlony double diffusion analysis with this antibody produced precipitin lines of identity with the purified rabbit lung enzyme and lung microsomes from pregnant and non-pregnant rabbits. No cross-reaction was seen with liver microsomes from rabbit or with purified pig liver enzyme.The tricyclic antidepressant drugs, imipramine and chlorpromazine, are not substrates for the rabbit lung enzyme, whereas they are rapidly oxidized by the pig liver enzyme.These results indicate that rabbit lung and liver flavin-containing monooxygenases differ in substrate specificity, are immunochemically distinct proteins, and may be different gene products.