Dynamical spectroscopy and microscopy of proteins in cells
Dynamical spectroscopy and microscopy of proteins in cells
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DOI：
10.1016/j.sbi.2021.02.001
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发表时间：
2021-10-01
影响因子：
6.8
通讯作者：
Pielak, Gary J.
中科院分区：
文献类型：
--
作者：
Gruebele, Martin;Pielak, Gary J.
随着对蛋白质如何折叠的深入了解，现在可以了解细胞内环境如何调节它们的折叠、结合和功能。在过去的几年里，通过细胞内NMR光谱和时间分辨荧光显微镜的结合，细胞动力学中快速（ns到s）的研究加速了。在这里，我们讨论了最近的工作和新兴的图片蛋白质表面不仅亲水涂层接口的溶剂蛋白质的核心和功能区域，但作为细胞中的关键组成部分，控制蛋白质的流动性，功能和通信与翻译后修饰。
With a strong understanding of how proteins fold in hand, it is now possible to ask how in-cell environments modulate their folding, binding and function. Studies accessing fast (ns to s) in cell dynamics have accelerated over the past few years through a combination of in-cell NMR spectroscopy and time-resolved fluorescence microscopies. Here, we discuss this recent work and the emerging picture of protein surfaces as not just hydrophilic coats interfacing the solvent to the protein's core and functional regions, but as critical components in cells controlling protein mobility, function and communication with post-translational modifications.