Assessment of renal maturity by assisted morphometry in autopsied fetuses
Assessment of renal maturity by assisted morphometry in autopsied fetuses
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DOI：
10.1016/j.earlhumdev.2006.01.013
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发表时间：
2006-11-01
影响因子：
2.5
通讯作者：
da Cunha Castro, Eumenia Costa
中科院分区：
文献类型：
--
作者：
dos Santos, Aline Mara;Fonseca Ferraz, Mara Lucia;da Cunha Castro, Eumenia Costa
背景：肾脏是一个依赖于GA而呈现出不同形态的器官；肾脏测量的经典参考文献是几年前建立的，目前尚不确定它们是否仍然准确。目的：应用计算机辅助形态计量学评估GA后20~40周内尸检胎儿的肾脏成熟度。研究设计：对67例死胎肾切片进行苏木精-伊红染色的形态计量学分析。结果：尸检资料中计算肾小球成熟层数的公式有可能调整为GA(Gln=0.212GA+0.0169)，这与文献中的公式(SR=0.709；P<；0.001)不同。对于生肾区，它随着GA的增加而减小，回归方程NZ=490.7-11.9xGA(p<；0.001；sr=-0.685)。结论：我们的数据有助于评估尸检胎儿的肾脏成熟度和GA，尤其是在自溶胎儿中，本研究中使用的参数仍然可以应用。对每个GA的成熟肾小球计数公式进行了修正，并增加了用于评估肾脏成熟度的新的形态计量学参数，提高了自然流产和尸检材料分析的精确度，改善了与临床表现和其他器官病理过程的相关性。(C)2006爱思唯尔爱尔兰有限公司。保留所有权利。
Background: The kidney is one organ which presents distinct morphology depending on GA; the classic references for kidney measurements were established several years ago and it is not certain if they are still accurate.Aim: To evaluate the renal maturity using a cornputer-assisted morphometry in autopsied fetuses within 20 to 40weeks of GA. Study design: Microscopic hematoxytin and eosin stained renal sections from 67 stillborns were utilized for the morphometric analyses.Results: It was possible to promote an adjustment in the formula used to calculate the number of mature glomeruli layers to the GA (GLN=0.212GA+0.0169) in autopsy material and it was different from the literature (Sr=0.709; p < 0.001). Regarding the nephrogenic zone, it decreases with the GA as confirmed by the regression equation NZ=490.7-(11.9xGA) (p < 0.001; Sr=-0.685).Conclusion: In conclusion, our data contribute to the evaluation of renal maturity and GA in autopsied fetuses, showing particular importance in autolyzed fetuses, in which the parameters used in the present study can still be applied. The correction of the formula for counting mature glomeruli layers for each GA and the addition of new morphometric parameters for the evaluation of renal maturity increase the precision for the analysis of spontaneous abortion and autopsy material, improving the correlation with pathological processes in clinical findings and in the other organs. (c) 2006 Elsevier Ireland Ltd. All rights reserved.