Dichelobacter nodosus, Fusobacterium necrophorum and the epidemiology of footrot
Dichelobacter nodosus, Fusobacterium necrophorum and the epidemiology of footrot
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DOI：
10.1016/j.anaerobe.2009.02.002
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发表时间：
2009-08-01
期刊：
影响因子：
2.3
通讯作者：
Zhou, Huitong
中科院分区：
文献类型：
--
作者：
Bennett, Grant;Hickford, Jon;Zhou, Huitong
羊步是一种使羊衰弱的疾病，导致羊跛行、产量损失和痛苦。为了研究该病的基本细菌学，在商业养殖场和非商业研究群中开展了一项调查，以比较有症状的足癣感染羊与健康的无症状羊的细菌学。在最初联系的80个农民中，14个收集了蹄拭子，并通过邮寄的方式归还。提取DNA后，采用物种特异性PCR方法鉴定每个拭子上是否存在结节双杆菌（d.n odosus）或坏死梭杆菌（e.n ophorum）。从有症状的羊身上采集的42份拭子中，17份拭子同时对尸吸虫和结瘤丁虫呈阳性，2份仅对尸吸虫呈阳性，2份仅对结瘤丁虫呈阳性，23份拭子对尸吸虫和结瘤丁虫均呈阴性。在50只健康无症状羊的拭子中，1只坏死性大肠杆菌阳性，49只坏死性大肠杆菌和F均阴性。这表明在一个牧区耕作系统中，necrophorum和d.s nodosus都与田间行走有关。如果这些细菌联系在一起并共同引起小跑，则在管理小跑爆发或维持隔离时可能需要考虑到这一点。2009爱思唯尔有限公司版权所有。
Footrot is a debilitating disease of sheep resulting in lameness, production losses and suffering. To study the basic bacteriology of the disease, a survey was initiated across commercial farms and non-commercial research flocks to compare the bacteriology of symptomatic footrot infected sheep with healthy asymptomatic sheep. Of the 80 farmers initially contacted, 14 collected hoof swabs and returned the swabs by post. Following DNA extraction, species-specific PCR was used to identify if Dichelobacter nodosus (D. nodosus) or Fusobacterium necrophorum (E necrophorum) species were present on each swab. Of the 42 swabs taken from symptomatic footrot infected sheep, 17 were positive for both F necrophorum and D. nodosus, two were positive for F necrophorum only, two for D. nodosus only and 23 swabs were negative for both F necrophorum and D. nod osus. Of the 50 swabs received from healthy asymptomatic sheep, one was positive for E necrophorum only and 49 were negative for both D. nodosus and F necrophorum. This suggests that both F. necrophorum and D. nodosus are linked to footrot in the field in a pastoral farming system. If these bacteria are linked together and collectively cause footrot, this may need to be considered when managing a footrot outbreak, or maintaining a quarantine. (C) 2009 Elsevier Ltd. All rights reserved.