Biopesticides offer an environmentally friendly solution for control of pine processionary moth ( Thaumetopoea wilkinsoni Tams) larvae and pupae in urban areas
Biopesticides offer an environmentally friendly solution for control of pine processionary moth ( Thaumetopoea wilkinsoni Tams) larvae and pupae in urban areas
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生物农药为控制城市地区的松树蛾（Thaumetopoea wilkinsoni Tams）幼虫和蛹提供了一种环保的解决方案
DOI：
10.1080/09583157.2020.1826905
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发表时间：
2020
影响因子：
1.4
通讯作者：
Güven Ö
中科院分区：
文献类型：
--
作者：
Güven Ö
在寻找新的生物防治方法来抑制松行蛾（PPM），Thaumetopoea wilkinsoniTams的过敏效应的框架下，无毒环保的昆虫病原真菌和精油被认为是化学杀虫剂的替代品。在田间和室内对照条件下，研究了精油和两株绿僵菌分离株（V275和ARSEF4556）对PPM幼虫和蛹的影响。巢内PPM幼虫和准备化蛹的后期幼虫对这两种菌株都高度敏感。在实验室中造成100%的死亡率，在田间试验中造成45-100%的死亡率。精油（生姜、桉树和迷迭香）对百万分之虫1、3、4龄幼虫的杀虫效果与剂量和虫龄成正比。姜油在水中稀释后，即使剂量低至1%和3% (v/v)，对一龄幼虫也有毒性。田间试验结果表明，3龄幼虫巢内注射精油可显著降低其死亡率（87.4%），但4龄幼虫的死亡率<5%。表面施用浓度为5%的所有精油可在一天内杀死一半的幼虫，而浓度为1%的精油可造成0%至20%的死亡率。在试验结束时，只有1%的生姜导致63%的死亡率。本研究表明姜油和m。brunneumestrain ARSEF 4556和V275在田间条件下具有控制PPM幼虫的潜力。
Within the framework of finding new biological control methods to suppress allergic effects of pine processionary moth (PPM),Thaumetopoea wilkinsoniTams, less toxic and environmentally friendly, entomopathogenic fungi and essential oils are considered to be an alternative to chemical insecticides. The aim of the present study was to investigate the effects of essential plant oils and two isolates ofMetarhizium brunneum(V275 and ARSEF4556) on PPM larvae and pupae under field and controlled laboratory conditions. PPM larvae inside the nest and late instar larvae preparing to pupate were highly susceptible to both strains ofM. brunneum,which caused 100% mortality in the laboratory and 45–100% mortality in field trials. The insecticidal effects of essential oils (ginger, eucalyptus and rosemary) on the first, third and fourth instar larvae of PPM were proportional to dosage and life stage of insect. Ginger oil diluted in water was toxic to first instar larvae even at a dose as low as 1% and 3% (v/v). Field trials showed that injection of essential oils into nests of third instar larvae caused significant mortality (87.4%) but <5% mortality of fourth instar larvae. Surface application of all essential oils at a concentration of 5% killed half of the larvae in one day, while 1% concentration caused from 0% to 20% mortality. Only 1% ginger caused 63% mortality at the end of the trial. This study shows that ginger oil andM. brunneumstrains ARSEF 4556 and V275 show promise for the control of PPM larvae under field conditions.