Traveled distance is a sensitive and accurate marker of motor dysfunction in a mouse model of multiple sclerosis.
Traveled distance is a sensitive and accurate marker of motor dysfunction in a mouse model of multiple sclerosis.
复制标题
在多发性硬化症的小鼠模型中，行进距离是运动功能障碍的灵敏而准确的标记。
DOI：
10.1155/2013/170316
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Takeuchi C
中科院分区：
文献类型：
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作者：
Takemiya T;Takeuchi C
多发性硬化症(MS)是一种常见的中枢神经系统疾病，与进行性身体损害有关。为了研究该病的发病机制，我们使用实验性自身免疫性脑脊髓炎(EAE)，用髓鞘少突胶质细胞糖蛋白35-55肽诱导MS的动物模型，并用EAE评分来衡量该病的瘫痪程度。在这里，我们比较了EAE评分和旅行距离，使用开场测试来评估EAE的进展。EAE评分采用6级瘫痪观察评分系统，行走距离由计算机检测到的自然探索行为自动轨迹分析获得。EAE小鼠的行走距离在EAE过程的第7天开始显著减少，此时EAE评分仍未反映变化。此外，在行走距离与第14天后EAE评分所测得的瘫痪之间的关系中，距离与评分之间存在较高的决定系数。结果表明，行走距离是EAE进展早期运动功能障碍的敏感指标，它反映了EAE瘫痪后运动功能障碍的程度。
Multiple sclerosis (MS) is a common central nervous system disease associated with progressive physical impairment. To study the mechanisms of the disease, we used experimental autoimmune encephalomyelitis (EAE), an animal model of MS. EAE is induced by myelin oligodendrocyte glycoprotein35–55 peptide, and the severity of paralysis in the disease is generally measured using the EAE score. Here, we compared EAE scores and traveled distance using the open-field test for an assessment of EAE progression. EAE scores were obtained with a 6-step observational scoring system for paralysis, and the traveled distance was obtained by automatic trajectory analysis of natural exploratory behaviors detected by a computer. The traveled distance of the EAE mice started to decrease significantly at day 7 of the EAE process, when the EAE score still did not reflect a change. Moreover, in the relationship between the traveled distance and paralysis as measured by the EAE score after day 14, there was a high coefficient of determination between the distance and the score. The results suggest that traveled distance is a sensitive marker of motor dysfunction in the early phases of EAE progression and that it reflects the degree of motor dysfunction after the onset of paralysis in EAE.