Host responses after acellular muscle basal lamina allografting used as a matrix for tissue engineered nerve grafts
Host responses after acellular muscle basal lamina allografting used as a matrix for tissue engineered nerve grafts
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DOI：
10.1097/00007890-200208150-00015
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发表时间：
2002-08-15
期刊：
影响因子：
6.2
通讯作者：
Keilhoff, G
中科院分区：
文献类型：
--
作者：
Fansa, H;Schneider, W;Keilhoff, G
背景神经间隙必须由自体神经移植物桥接，自体神经移植物作为支架，由促进再生的活的雪旺细胞组成。由于需要免疫抑制，同种异体神经移植仅限于特殊情况。或者，周围神经的组织工程集中于将培养的雪旺细胞植入合适的支架中。我们建立了雪旺细胞和无细胞肌肉基膜的移植物。这些移植物提供了与自体神经移植物相当的再生。采用大鼠模型（DALEW.1W品系），本研究通过评估细胞反应主要组织相容性（MHC）I类和II类，淋巴细胞，巨噬细胞来评估宿主对脱细胞肌肉同种异体移植物的反应。将结果与未处理的同种异体肌肉移植物进行比较。宏观上，未处理的肌肉表现出强烈的炎症反应，作为排斥反应的标志，而无细胞的肌肉在移植物的外周仅提供轻微的反应。未处理的肌肉移植后，MHC I和II的表达和CD 4/CD 8阳性细胞和巨噬细胞的侵袭是明显的。无细胞移植后，这些参数仅出现中度反应。脱细胞肌肉移植物并非完全没有细胞反应;然而，反应被认为是中度的，并且仅位于外周。迄今为止，代表神经内膜管样结构并允许雪旺细胞充分粘附和轴突再生的合成支架不可用。对脱细胞肌肉同种异体移植物的反应降低至少为进一步的实验提供了基础。
Background. A nerve gap must be bridged by autologous nerve grafts that serve as scaffold and consist of viable Schwann cells that promote regeneration. Owing to the necessary immunosuppression, nerve allografts remain limited to special cases. Alternatively, tissue engineering of peripheral nerves focuses on the implantation of cultured Schwann cells into suitable scaffolds. We established grafts from Schwann cells and basal lamina from acellular muscles. These grafts offer a regeneration that is comparable to autologous nerve grafts.Methods. Using a rat model (DALEW.1W strain), the present study evaluates the host response to acellular muscle allografts by assessing cellular reaction major histocompatability (MHC) class I and II, lymphocytes, macrophages. The results were compared to untreated muscle allografts.Results. Macroscopically, the untreated muscles showed a strong inflammatory reaction as a sign of rejection, whereas the acellular muscle offered only minor reactions in the periphery of the graft. Expression of MHC I and II and invasion of CD4/CD8 positive cells and macrophages was pronounced after grafting the untreated muscles. Only a moderate reaction was noted for these parameters after acellular grafting.Conclusions. The acellular muscle graft is not completely free of cellular response; however the reaction is considered to be moderate and is located only in the periphery. To date, synthetic scaffolds that represent endoneurial tube-like structures and allow sufficient adhesion of Schwann cells and axonal regeneration are not available. The decreased response to acellular muscle allografts offers at least a basis for further experiments.