PAB 2000 SPECIFICALLY RECOGNIZES THE LARGE-T AND SMALL-T PROTEINS OF JC VIRUS
PAB 2000 SPECIFICALLY RECOGNIZES THE LARGE-T AND SMALL-T PROTEINS OF JC VIRUS
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DOI：
10.1016/0168-1702(92)90136-w
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发表时间：
1992-09-15
期刊：
影响因子：
5
通讯作者：
FRISQUE, RJ
中科院分区：
文献类型：
--
作者：
BOLLAG, B;FRISQUE, RJ
制备了一种单克隆抗体PAb 2000，可识别JC病毒（JCV）的大T蛋白，而不能识别相关多瘤病毒BK病毒（BKV）和SV40的相应蛋白。PAb 2000结合的表位定位于该多功能蛋白的氨基末端81个氨基酸。正如之前对几种结合SV40大T抗原相似区域的单克隆抗体所观察到的那样，PAb 2000被发现与小T抗原和大T抗原的变性形式相互作用。该单克隆抗体识别T蛋白亚群，该亚群的丰度在不同种类的JCV转化细胞中有所不同。首个JCV T抗原特异性单克隆抗体PAb 2000的上市，将有助于JCV癌蛋白的纯化和生化表征。
A monoclonal antibody, PAb 2000, has been derived which recognizes the large T protein of JC virus (JCV), but not the corresponding proteins of the related polyomaviruses BK virus (BKV) and SV40. The epitope bound by PAb 2000 was localized to the amino-terminal 81 amino acids of this multifunctional protein. As observed previously with several monoclonal antibodies that bind a similar region of SV40 large T antigen, PAb 2000 was found to interact with the small t antigen and the denatured form of large T antigen. This monoclonal antibody recognized a subpopulation of T protein, the abundance of which varied in different species of cells transformed by JCV. The availability of PAb 2000, the first JCV T antigen-specific monoclonal antibody, will facilitate the purification and biochemical characterization of the JCV oncoproteins.