Serotonin release in the subfornical organ area induced by sodium and water intake in the rat
Serotonin release in the subfornical organ area induced by sodium and water intake in the rat
复制标题
钠和水摄入引起大鼠穹窿下器官区域血清素释放
DOI：
10.1016/j.physbeh.2016.04.037
复制
发表时间：
2016
影响因子：
2.9
通讯作者：
TAKAHASHI makoto・TANAKA junichi
中科院分区：
文献类型：
--
作者：
Shirahama;N.;Watanabe;S.;Nakaya;N. and Mori;Y.;田中淳一・高橋眞琴;佐々木玲子;渡邉志，白濱成希;TAKAHASHI makoto・TANAKA junichi;渡邉志，白濱成希，松本有二，塚本博之，中谷直史，森幸男;TAKAHASHI makoto・HAYASHI yasushi・TANAKA junichi;鶴田利郎，白濱成希，森幸男，中谷直史，塚本博之，松本有二，渡邉志;渡邉志，白濱成希，池上郁子，塚本博之，松本有二，中谷直史，森幸男;TAKAHASHI makoto・TANAKA junichi
以往的研究表明，皮层下器官（SFO）参与了大鼠钠摄入量的调节。为了阐明SFO中的5-羟色胺能机制是否参与调节系统，本研究研究了钠和水摄入对自由运动大鼠皮层下器官（SFO）中5-羟色胺（5-hydroxytryptamine, 5-HT）释放的影响。皮下注射利尿剂速尿（FURO, 10 mg/kg）和血管紧张素转换酶抑制剂卡托普利（captopril, 5 mg/kg），诱导小鼠摄入0.3 M NaCl和水，并采用脑内微透析技术测定SFO区域5-羟色胺及其代谢物5-羟基吲哚乙酸（5- hiaa）的细胞外浓度。FURO和CAP联合处理可显著降低SFO区5-HT和5-HIAA水平。0.3 M NaCl和水分处理显著提高了SFO区5-HT和5-HIAA浓度。在远离SFO的部位，没有观察到FURO和CAP处理或水和NaCl摄入引起的5-HT和5-HIAA水平的显著变化。这些结果表明，SFO中的血清素能机制可能对钠食欲和口渴的控制很重要。
Previous studies have shown that the subfornical organ (SFO) is involved in the modulation of sodium intake in rats. To clarify whether serotonergic mechanisms in the SFO participate in the modulatory system, the present study was carried out to examine the effects of sodium and water intake on serotonin (5-hydroxytryptamine, 5-HT) release in the subfornical organ (SFO) in freely moving rats. The ingestion of 0.3 M NaCl and water was induced by subcutaneous injections of the diuretic furosemide (FURO, 10 mg/kg) and the angiotensin converting enzyme inhibitor captopril (CAP, 5 mg/kg), and extracellular concentrations of 5-HT and its metabolite 5-hydroxyindoleacetic acid (5-HIAA) in the region of the SFO were measured using intracerebral microdialysis techniques. The combined treatment with FURO and CAP elicited significant decreases in the 5-HT and 5-HIAA levels in the SFO area. The 0.3 M NaCl and water intake caused by the treatment significantly enhanced the 5-HT and 5-HIAA concentrations in the SFO area compared with the basal levels. No significant changes in the 5-HT and 5-HIAA levels caused by either the FURO and CAP treatment or water and NaCl intake were observed in the sites away from the SFO. These results suggest that the serotonergic mechanism in the SFO may be important for the control of sodium appetite and thirst.