Ralstonia taiwanensis sp nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient
Ralstonia taiwanensis sp nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient
复制标题
DOI：
10.1099/00207713-51-5-1729
复制
发表时间：
2001-09-01
影响因子：
2.8
通讯作者：
Vandamme, P
中科院分区：
文献类型：
--
作者：
Chen, WM;Laevens, S;Vandamme, P
通过16 S rDNA序列分析、DNA-DNA杂交、DNA碱基比测定、16 S rDNA扩增、全细胞蛋白分析和广泛的生化特性分析，对8株含羞草根瘤菌和1株囊性纤维化患者痰液中分离物进行了多相分类学研究。所有9个分离物被分类为一个新的罗尔斯通氏菌种，并建议将其命名为Ralstonia taiwanensis sp. nov.。模式菌株为LMG 19424（T）（= CCUG 44338（T））。R. taiwanensis有效地使含羞草属植物种化，并且是已知的第一个能够形成根瘤和固氮的β-变形杆菌。
A polyphasic taxonomic study, including 16S rDNA sequence analysis, DNA-DNA hybridizations, DNA base ratio determinations, amplified 16S rDNA restriction analysis, whole-cell protein analyses and extensive biochemical characterization, was conducted to clarify the relationships of eight isolates from root nodules of Mimosa species and one isolate from sputum of a cystic fibrosis patient. All nine isolates were classified as a novel Ralstonia species, for which the name Ralstonia taiwanensis sp. nov. is proposed. The type strain is LMG 19424(T) (= CCUG 44338(T)). R. taiwanensis effectively nodulated the Mimosa species and is the first beta -proteobacterium that is known to be capable of root nodule formation and nitrogen fixation.