Time course of hypertrophic and ultrastructural responses of rat stomach enterochromaffin-like cells to sustained hypergastrinemia
Time course of hypertrophic and ultrastructural responses of rat stomach enterochromaffin-like cells to sustained hypergastrinemia
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大鼠胃肠嗜铬样细胞对持续性高胃泌素血症的肥大和超微结构反应的时程
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发表时间：
1996
影响因子：
3.6
通讯作者：
R. Håkanson
中科院分区：
文献类型：
--
作者：
Duan Chen;Chun;A. Nylander;R. Håkanson
摘要：之前，我们研究了胃泌素短期（几分钟至几小时）和长期（几周至几个月）刺激对大鼠胃泌酸粘膜中产生组胺的肠嗜铬样（ECL）细胞的影响。本研究检查了自由喂养的大鼠的 ECL 细胞在几小时至四个星期的时间内对持续高胃泌素血症的反应。持续皮下输注人 Leu15-胃泌素-17 诱发持续性高胃泌素血症。在整个研究过程中监测组氨酸脱羧酶（HDC）活性和泌酸粘膜中的组胺浓度。对电子显微照片中的 ECL 电池轮廓进行平面分析。 HDC 活性在前两天内增加了 4 倍。随后，它一直处于平台期。胃泌素的作用下，组胺浓度增加 2 至 3 倍。组胺的上升速度慢于 HDC 活性的上升速度。组胺浓度在任何时间点都没有降低。高胃泌素血症 4 天后，ECL 细胞体积增大，2 周后达到最大细胞轮廓面积，并在研究期间保持增大状态。 1 天后分泌小泡数量减少，此后逐渐恢复到刺激前的值；在 6 天的观察期内，它们的体积密度仍然降低。高胃泌素血症1天后开始出现空泡，其数量和体积密度增加，4天后达到最大。微泡的数量和体积密度增加，并在高胃泌素血症 2 天后趋于稳定。高胃泌素血症 4 天后，每个细胞轮廓的颗粒数量不受影响，但其体积密度大大降低（反映 ECL 细胞肥大）。目前的研究结果确定了 ECL 细胞响应持续高胃泌素血症而激活的时间过程，时间跨度为几小时到四个星期；大约一周后，高活动水平的新“稳定状态”已经建立。
Abstract.Previously, we have investigated the effects of short-term (minutes to hours) and long-term (weeks to months) stimulation with gastrin on the histamine-producing enterochromaffin-like (ECL) cells in the oxyntic mucosa of rat stomach. The present study examines the response of the ECL cells of freely fed rats to sustained hypergastrinemia over a time span of a few hours to four weeks. Sustained hypergastrinemia was induced by the continuous subcutaneous infusion of human Leu15-gastrin-17. The histidine decarboxylase (HDC) activity and histamine concentration in the oxyntic mucosa were monitored throughout the study. ECL cell profiles in electron micrographs were analysed planimetrically. The HDC activity displayed a 4-fold increase within the first two days. Subsequently, it remained at a plateau. The histamine concentration increased 2- to 3-fold in response to gastrin. The rise in histamine was slower than the rise in HDC activity. At no time point was there a reduced concentration of histamine. The ECL cells increased in size after 4 days of hypergastrinemia, reaching a maximum cell profile area after 2 weeks and remaining enlarged for the duration of the study. The secretory vesicles were reduced in number after 1 day, returning gradually to the pre-stimulation value thereafter; their volume density remained reduced during the 6-day observation period. Vacuoles started to appear after 1 day of hypergastrinemia and their number and volume density increased, reaching a maximum after 4 days. The number and volume density of the microvesicles increased and plateaued after 2 days of hypergastrinemia. The number of granules per cell profile was unaffected but their volume density was greatly reduced after 4 days of hypergastrinemia (reflecting the ECL cell hypertrophy). The present findings establish the time course of activation of the ECL cells in response to sustained hypergastrinemia over a time span of a few hours to four weeks; a new ”steady state” situation at a high level of activity has been established after about a week.
大鼠肠嗜铬样细胞分泌组胺。
影响因子：
29.4
作者：
Prinz,C;Kajimura,M;Scott,DR;Mercier,F;Helander,HF;Sachs,G
通讯作者：
Sachs,G