Urinary excretion of podocytes in patients with diabetic nephropathy
Urinary excretion of podocytes in patients with diabetic nephropathy
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DOI：
10.1093/ndt/15.9.1379
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发表时间：
2000-09-01
影响因子：
6.1
通讯作者：
Koide, H
中科院分区：
文献类型：
--
作者：
Nakamura, T;Ushiyama, C;Koide, H
背景。肾小球肾炎患儿尿液沉积物中足细胞的检测表明足细胞受到严重损伤。本研究的目的是确定足细胞是否存在于伴有或不伴有肾病的成年糖尿病患者的尿液沉积物中，以及曲多拉普利是否对足细胞损伤有效。方法：对50例糖尿病患者（正常蛋白尿10例、微量蛋白尿15例、大量蛋白尿15例、慢性肾功能衰竭10例）和10例健康对照进行研究。利用存在于足细胞表面的抗足霉素单克隆抗体对尿足细胞进行免疫荧光检测。此外，我们研究了所有患者血浆金属蛋白酶(MMP)-9的浓度。结果：健康对照组、尿白蛋白尿正常的糖尿病患者和慢性肾功能衰竭的糖尿病患者均无足细胞。8例伴有微量白蛋白尿的糖尿病患者（53%）和12例伴有大量白蛋白尿的糖尿病患者（80%）尿液中检测到足细胞。大量白蛋白尿患者尿中足细胞数量明显多于微量白蛋白尿患者(P
Background. Detection of podocytes in the urinary sediments of children with glomerulonephritis has been shown to indicate severe injury to the podocytes. The aim of the present study was to determine whether podocytes are present in the urine sediments of adult patients with diabetes with and without nephropathy and whether trandolapril is effective for podocyte injury.Methods, Fifty diabetic patients (10 with normoalbuminuria, 15 with microalbuminuria, 15 with macroalbuminuria and 10 with chronic renal failure) and 10 healthy controls were studied. Urinary podocytes were examined by immunofluorescence using monoclonal antibodies against podocalyxin, which is present on the surface of podocytes. In addition, we studied plasma metalloproteinase (MMP)-9 concentrations in all patients.Results, Urinary podocytes were absent in healthy controls, diabetic patients with normoalbuminuria and diabetic patients with chronic renal failure. Podocytes were detected in the urine of eight diabetic patients with microalbuminuria (53%) and of 12 patients with macroalbuminuria (80%). The number of podocytes in the urine of patients with macroalbuminuria was significantly greater than in patients with microalbuminuria (P