Copper deficiency anemia: altered red blood cell lipids and viscosity in rats.
Copper deficiency anemia: altered red blood cell lipids and viscosity in rats.
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DOI：
10.1093/ajcn/48.3.637
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发表时间：
1988-09
期刊：
影响因子：
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通讯作者：
S. Jain;D. Williams
中科院分区：
文献类型：
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作者：
S. Jain;D. Williams
为了研究膜脂异常及其在缺铜性贫血中的可能作用，我们研究了 3 周龄时喂食含有 1.1 mg Cu/kg（缺乏 Cu-）和 6.0 mg Cu/kg（补充 Cu+）饮食的大鼠的脂质、渗透脆性和红细胞 (RBC) 粘度，持续 9 周。数据显示，与 Cu+ 大鼠相比，Cu- 大鼠每个细胞的胆固醇增加了 43%，磷脂增加了 38%。红细胞脂质薄层色谱显示，与 Cu+ (0.29 +/- 0.19%) 大鼠相比，Cu- 大鼠中磷脂-丙二醛加合物的量增加（总磷脂的 0.70 +/- 0.35% [平均值 +/- SD]），表明 Cu- 红细胞中膜脂过氧化增加。铜鼠红细胞的渗透脆性较低。在剪切速率为 23、11.5 和 5.75 剪切/秒时，Cu- 大鼠的红细胞粘度显着高于 Cu+ 大鼠，并且在剪切速率 115 和 46 剪切/秒时相似。脂质负荷引起的细胞粘度增加以及脂质过氧化引起的膜成分交联可能与铜缺乏时红细胞存活率降低和贫血有关。
To investigate membrane lipid abnormalities and their possible role in copper deficiency anemia, we studied lipids, osmotic fragility, and viscosity of red blood cells (RBCs) of rats fed diets containing 1.1 mg Cu/kg (deficient, Cu-) and 6.0 mg Cu/kg (supplemented, Cu+) at age 3 wk for 9 wk. Data show an increase in cholesterol of 43% and in phospholipids of 38% per cell in Cu- compared with Cu+ rats. Thin-layer chromatography of RBC lipids showed an increased amount of phospholipid-malonyldialdehyde adduct in Cu- (0.70 +/- 0.35% [means +/- SD] of total phospholipids) compared with Cu+ (0.29 +/- 0.19%) rats, suggesting increased membrane lipid peroxidation in Cu- RBC. Osmotic fragility of RBCs from Cu- rats was lower. RBC viscosity was significantly higher in Cu- than in Cu+ rats at shear rates of 23, 11.5, and 5.75 shear/s and was similar at shear rates 115 and 46 shear/s. Increased cell viscosity caused by lipid loading and crosslinking of membrane components caused by lipid peroxidation may have a role in the reduced RBC survival and anemia in Cu deficiency.