Converting a Natural Protein Compartment into a Nanofactory for the Size-Constrained Synthesis of Antimicrobial Silver Nanoparticles
Converting a Natural Protein Compartment into a Nanofactory for the Size-Constrained Synthesis of Antimicrobial Silver Nanoparticles
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DOI：
10.1021/acssynbio.6b00117
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发表时间：
2016-12-01
影响因子：
4.7
通讯作者：
Silver, Pamela A.
中科院分区：
文献类型：
--
作者：
Giessen, Tobias W.;Silver, Pamela A.
工程生物系统广泛用于生产高价值和商品有机物。另一方面，大多数无机纳米材料仍然通过化学途径合成。通过设计细胞隔室，可以在环境可持续的条件下生产功能性纳米结构。微胶囊是一类新型的微生物纳米区室，在纳米生物技术中具有广阔的应用前景。在这里，我们工程师Thermotoga maritima的EncTm产生一个设计的隔室的尺寸限制合成的银纳米粒子（银纳米粒子）。这些银纳米颗粒表现出均匀的形状和尺寸分布以及长期稳定性。环境水性条件可用于Ag NP合成，而不需要添加还原剂或溶剂。合成的蛋白质涂层或无壳银纳米颗粒的抗微生物活性上级硝酸银和柠檬酸盐覆盖的银纳米颗粒。这项研究建立了一个工程化的平台，合成生物功能纳米材料。
Engineered biological systems are used extensively for the production of high value and commodity organics. On the other hand, most inorganic nanomaterials are still synthesized via chemical routes. By engineering cellular compartments, functional nanoarchitectures can be produced under environmentally sustainable conditions. Encapsulins are a new class of microbial nanocompartments with promising applications in nanobiotechnology. Here, we engineer the Thermotoga maritima encapsulin EncTm to yield a designed compartment for the size-constrained synthesis of silver nanoparticles (Ag NPs). These Ag NPs exhibit uniform shape and size distributions as well as long-term stability. Ambient aqueous conditions can be used for Ag NP synthesis, while no reducing agents or solvents need to be added. The antimicrobial activity of the synthesized protein-coated or shell-free Ag NPs is superior to that of silver nitrate and citrate-capped Ag NPs. This study establishes encapsulins as an engineerable platform for the synthesis of biogenic functional nanomaterials.