Characteristics of acid-sensing ion channel currents in male rat muscle dorsal root ganglion neurons following ischemia/reperfusion.
Characteristics of acid-sensing ion channel currents in male rat muscle dorsal root ganglion neurons following ischemia/reperfusion.
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缺血/再灌注后，雄性大鼠背部神经节神经元中酸性离子通道电流的特征。
DOI：
10.14814/phy2.15654
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发表时间：
2023-03
影响因子：
2.5
通讯作者：
中科院分区：
文献类型：
--
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外周动脉疾病（PAD）增加运动期间肌肉传入神经激活反射交感神经和血压反应（称为运动加压反射）。然而，确切的信号通路导致过度的自主反应在PAD是不确定的。考虑到肢体缺血/再灌注（I/R）是PAD的一个特征，我们测定了在后肢I/R和PAD缺血条件下肌肉背根神经节（DRG）神经元酸敏感离子通道（ASIC）电流的特征。特别是，我们检查了两个不同亚群中的ASIC电流，isolectin B4阳性和B4阴性（IB 4+和IB 4-）肌肉DRG神经元，与胶质细胞系源性神经营养因子和神经生长因子相关。结果，在IB 4 −肌DRG神经元中观察到ASIC 1a和ASIC 3样电流，ASIC 3样电流的百分比更高。Hindimb I/R和缺血并没有改变IB 4+和IB 4 −肌DRG神经元中pH 6.7激活的ASIC 1a和ASIC 3电流的分布;然而，I/R改变了pH 5.5的IB 4+肌DRG神经元中ASIC 3电流的分布，但在IB 4 −神经元中没有改变。此外，I/R和缺血放大了IB 4 −肌DRG神经元中ASIC 3样电流的密度。我们的研究结果表明，肌肉传入神经的选择性亚群时，应考虑ASIC信号通路的研究，以确定运动加压反射在PAD。缺血和缺血-再灌注影响肌肉感觉神经元中的酸敏感离子通道。结果表明，酸敏感离子通道3型在外周动脉疾病的细胞水平上调节运动升压反射中起主要作用。
Peripheral artery diseases (PAD) increases muscle afferent nerve‐activated reflex sympathetic nervous and blood pressure responses during exercise (termed as exercise pressor reflex). However, the precise signaling pathways leading to the exaggerated autonomic responses in PAD are undetermined. Considering that limb ischemia/reperfusion (I/R) is a feature of PAD, we determined the characteristics of acid‐sensing ion channel (ASIC) currents in muscle dorsal root ganglion (DRG) neurons under the conditions of hindlimb I/R and ischemia of PAD. In particular, we examined ASIC currents in two distinct subpopulations, isolectin B4‐positive, and B4‐negative (IB4+ and IB4−) muscle DRG neurons, linking to glial cell line‐derived neurotrophic factor and nerve growth factor. In results, ASIC1a‐ and ASIC3‐like currents were observed in IB4− muscle DRG neurons with a greater percentage of ASIC3‐like currents. Hindimb I/R and ischemia did not alter the distribution of ASIC1a and ASIC3 currents with activation of pH 6.7 in IB4+ and IB4− muscle DRG neurons; however, I/R altered the distribution of ASIC3 currents in IB4+ muscle DRG neurons with pH 5.5, but not in IB4− neurons. In addition, I/R and ischemia amplified the density of ASIC3‐like currents in IB4− muscle DRG neurons. Our results suggest that a selective subpopulation of muscle afferent nerves should be taken into consideration when ASIC signaling pathways are studied to determine the exercise pressor reflex in PAD. Ischemia and ischemia–reperfusion affect acid‐sensing ion channels in muscle sensory neurons. The results suggest that acid‐sensing ion channel type 3 plays a major role in regulating the exercise pressor reflex at the cellular level in peripheral artery disease.
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DOI：
10.1152/ajpheart.00257.2020
发表时间：
2020-07-01
影响因子：
4.8
作者：
Ducrocq, Guillaume P.;Kim, Joyce S.;Kaufman, Marc P.
通讯作者：
Kaufman, Marc P.
影响因子：
2.8
作者：
de Liefde, Inge I.;Hoeks, Sanne E.;Poldermans, Don
通讯作者：
Poldermans, Don
影响因子：
11.4
作者：
Deval, Emmanuel;Noel, Jacques;Lingueglia, Eric
通讯作者：
Lingueglia, Eric
影响因子：
25
作者：
Immke, DC;McCleskey, EW
通讯作者：
McCleskey, EW
影响因子：
5.5
作者：
Hayes, Shawn G.;Kindig, Angela E.;Kaufman, Marc P.
通讯作者：
Kaufman, Marc P.