A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore.
A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore.
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DOI：
10.1016/j.talanta.2012.02.025
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发表时间：
2012-05
期刊：
影响因子：
6.1
通讯作者：
Daisuke Seto;T. Maki;N. Soh;K. Nakano;R. Ishimatsu;T. Imato
中科院分区：
文献类型：
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作者：
Daisuke Seto;T. Maki;N. Soh;K. Nakano;R. Ishimatsu;T. Imato
描述了一种使用与 Fe2+ 离子复合的钙黄绿素蓝 (CB) 作为化学传感器的新型多巴胺荧光测定法。 CB-Fe2+ 复合物的 CB 产生的荧光被 Fe2+ 离子猝灭。当多巴胺添加到 CB–Fe2+ 复合物溶液中时，CB 和多巴胺之间发生配体交换反应，形成多巴胺–Fe2+ 复合物，从而恢复 CB 的荧光。发现波长 440 nm（激发波长 340 nm）处的荧光强度与添加到 CB-Fe2+ 络合物溶液中的多巴胺浓度成正比，从而可以定量测定多巴胺。在其他儿茶酚胺和相关化合物存在下，多巴胺的选择性良好。基于CB和多巴胺之间的配体交换反应的平衡，成功地模拟了使用实验数据确定的多巴胺的校准曲线。工作范围为 50μM 至 1mM，检测限和量化限分别约为 10μM 和 50μM。与酶联免疫吸附测定（ELISA）等传统方法相比，该测定简单且经济。
A novel fluorimetric assay for dopamine using calcein blue (CB) complexed with Fe2+ion as a chemical sensor is described. The fluorescence arising from CB of the CB–Fe2+complex is quenched by the Fe2+ion. When dopamine is added to a solution of the CB–Fe2+complex, a dopamine–Fe2+complex is formed as the result of a ligand exchange reaction between CB and dopamine which permits the fluorescence from CB to be recovered. The fluorescence intensity at the wavelength of 440nm (at the excitation wavelength of 340nm) was found to be proportional to the concentration of the dopamine added to the CB–Fe2+complex solution, which permits dopamine to be quantitatively determined. The selectivity for dopamine in the presence of other catecholamines and related compounds was good. The calibration curve for dopamine, determined using experimental data was successfully simulated based on the equilibrium of the ligand exchange reaction between CB and dopamine. The working range is from 50μM to 1mM and the limit of detection and limit of quantization are ca 10μM and 50μM, respectively. The assay is simple and economical, compared with conventional methods such as an enzyme-linked immunosorbent assay (ELISA).