Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J
Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J
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自噬靶向 DNA 疫苗针对禽白血病病毒 J 亚型的功效
DOI：
10.1016/j.vaccine.2016.12.034
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发表时间：
2017-02-01
期刊：
影响因子：
5.5
通讯作者：
Xie, Qingmei
中科院分区：
文献类型：
--
作者：
Dai, Zhenkai;Huang, Jianfei;Xie, Qingmei
J亚群禽白血病病毒（ALV-J）的感染可导致鸡的肿瘤性疾病，给家禽业造成重大的经济损失。最近的报道已经确定ALV-J对自噬的抑制作用，自噬是涉及先天性和适应性免疫的过程。受自噬和免疫之间的这种联系的启发，我们开发了一种新的抗ALV-J的DNA疫苗，其中包括共同施用雷帕霉素以刺激自噬。为了检测所开发的原型疫苗的效力，将5个实验组的7日龄鸡分别用载体pVAX 1-gp 85、pVAX 1-gp 85-LC 3、pVAX 1-gp 85 + rapamycin和pVAX 1-gp 85-LC 3 + rapamycin通过电穿孔免疫3次，间隔3周。然后，我们测试了他们的抗体滴度，细胞因子水平和细胞免疫反应。还检查了原型疫苗针对ALV-J GD 1109毒株攻击的免疫保护效力。结果表明：pVAX 1-gp 85-LC 3与雷帕霉素联合免疫鸡后，抗体效价最高，IL-2、IL-10和IFN-γ表达增强，CD 3 + CD 8 + T淋巴细胞比例最高。基于我们的研究结果，我们认为刺激自噬可以提高DNA疫苗的效力，并且我们的DNA疫苗显示出作为ALV-J候选疫苗的潜力。这项研究为开发ALV-J疫苗提供了一种新的策略。（C）2016 Elsevier Ltd.版权所有。
Infection with the avian leukosis virus subgroup J (ALV-J) can lead to neoplastic disease in chickens, inflicting significant economic losses to the poultry industry. Recent reports have identified inhibitory effects of ALV-J on autophagy, a process involving in innate and adaptive immunity. Inspired by this connection between autophagy and immunity, we developed a novel DNA vaccine against ALV-J which includes co-administration of rapamycin to stimulate autophagy. To measure the efficacy of the developed prototype vaccine, five experimental groups of seven-day-old-chickens was immunized three times at three-week intervals respectively with vector, pVAX1-gp85, pVAX1-gp85-LC3, pVAX1-gp85 + rapamycin and pVAX1-gp85-LC3 + rapamycin through electroporation. We then tested their antibody titers, cytokine levels and cellular immune responses. The immunoprotective efficacy of the prototype vaccines against the challenge of the ALV-J GD1109 strain was also examined. The results showed that the combination of pVAX1-gp85-LC3 and rapamycin was able to induce the highest antibody titers, and enhance interleukin(IL)-2, IL-10 and interferon (IFN)-gamma expression, and the chickens immunized with the combination of pVAX1-gp85-LC3 and rapamycin showed the highest percentage of CD3+ CD8+ T lymphocytes. Based on our results, we suggest that stimulating autophagy can improve the efficacy of DNA vaccines and that our DNA vaccine shows the potential of being a candidate vaccine against ALV-J. This study provides a novel strategy for developing vaccines against ALV-J. (C) 2016 Elsevier Ltd. All rights reserved.