Improved viability of probiotics encapsulated in soybean protein isolate matrix microcapsules by coacervation and cross-linking modification
Improved viability of probiotics encapsulated in soybean protein isolate matrix microcapsules by coacervation and cross-linking modification
复制标题
通过凝聚和交联改性提高封装在大豆分离蛋白基质微胶囊中的益生菌的活力
DOI：
10.1016/j.foodhyd.2023.108457
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发表时间：
2023-01-10
影响因子：
10.7
通讯作者：
Liu, Huan
中科院分区：
文献类型：
--
作者：
Hu, Ronghai;Dong, Dejun;Liu, Huan
以大豆分离蛋白（SPI）为基质，采用凝聚法、交联法及二者结合法制备益生菌微胶囊，并研究喷雾干燥、体外消化、加热、紫外线照射和贮藏等对益生菌存活的影响。将益生菌分离物植物乳杆菌550在与果胶（PEC）、转氨酶（TGase）或它们的组合的SPI溶液中喷雾干燥。所有样品的成活率都很高，含水量都很低。采用SPI-PEC凝聚法或TGase交联法制备的微胶囊在胃消化、热/紫外处理和储存过程中均获得了良好的细胞存活率，然而，这两种改性方法的组合产生的微胶囊具有较低的交联度和松散的包装结构，这对应于胃消化和储存过程中对细胞活力的显著负面影响。在所测试的配方中，SPI-PEC微粒对益生菌细胞提供了最好的保护，因为果胶具有良好的热稳定性和消化能力，可以填充SPI基质中存在的裂缝和空隙，并在加工过程中形成氢键以稳定细胞膜。综上所述，本研究表明，具有高结构密度和高交联度的SPI基益生菌微胶囊可以对不利的外部环境提供更好的保护。
The objective of this research was to encapsulate probiotic bacteria based on soy protein isolate (SPI) matrix using either coacervation, cross-linking modification or their combination, and investigate the influences on the survival of probiotic bacteria during spray drying, in vitro gastrointestinal digestion, heating, UV irradiation and storage. A probiotic isolate Lactobacillus plantarum 550 was spray dried in SPI solution combined with either pectin (PEC), transglutaminase (TGase) or their combination. High survival rate and low moisture content were obtained for all the samples. Microcapsules fabricated using either SPI-PEC coacervation or TGase induced crosslinking obtained well cell survival during gastric digestion, heat/UV treatment and storage, however, combination of these two modification methods generated microcapsules with lower cross-linking degree and loose package structure corresponding with significant negative effects to the cell viability during gastric digestion and storage. Among the formulations tested, SPI-PEC microparticles provided the best protection to the probiotic cells, as pectin own well thermal stability and digestion resistibility, could fill the cracks and voids existed in SPI matrix and form hydrogen bond to stabilize cell membrane during processing. In conclusion, the current study demonstrated that SPI based probiotic microcapsules with high structural density and cross-linking degree may provide better protection to adverse external environment.