Differently Expression Analysis and Function Prediction of Long Non-coding RNAs in Duck Embryo Fibroblast Cells Infected by Duck Tembusu Virus
Differently Expression Analysis and Function Prediction of Long Non-coding RNAs in Duck Embryo Fibroblast Cells Infected by Duck Tembusu Virus
复制标题
鸭天宝苏病毒感染鸭胚胎成纤维细胞中长链非编码RNA的差异表达分析及功能预测
DOI：
10.3389/fimmu.2020.01729
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发表时间：
2020-08-04
影响因子：
7.3
通讯作者：
Diao, Youxiang
中科院分区：
文献类型：
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作者：
Lin, Yun;Yang, Jing;Diao, Youxiang
鸭坦布苏病毒（DTMUV）是引起鸭产蛋下降综合征的病原，给养鸭业造成了巨大的经济损失。DTMUV感染引起宿主细胞的深刻变化，包括转录组和蛋白质组。然而，lncRNA的表达谱和lncRNA的生物学功能尚未被揭示。因此，DTMUV被用于扩增鸭胚成纤维细胞（DEF）用于高通量RNA测序（RNA-Seq）。结果表明，在感染后12和24 h（hpi）分别鉴定出34和339个不同表达的lncRNA。为了分析它们的生物学功能，寻找顺式的靶基因并构建调控网络。京都基因和基因组百科全书（KEGG）富集分析表明，靶基因与免疫系统、信号分子和相互作用、内分泌系统和信号传导密切相关。选择差异表达的lncRNA并通过定量实时聚合酶链反应（RT-qPCR）进行验证。我们的研究首次分析了DTMUV感染后DEF中全面的lncRNA表达谱。该分析为lncRNA在基因调控和DTMUV感染中的重要作用提供了一种观点。
Duck Tembusu virus (DTMUV), the causative agent of egg-drop syndrome, has caused substantial economic losses to duck industry. DTMUV infection leads to profound changes of host cells, including transcriptome and proteome. However, the lncRNA expression profile and the biological function of lncRNA have not been revealed. Therefore, DTMUV was used to inoculate duck embryo fibroblast cells (DEFs) for high-throughput RNA-sequencing (RNA-Seq). The results showed that 34 and 339 differently expressed lncRNAs were, respectively, identified at 12 and 24 h post-infection (hpi). To analyze their biological functions, target genes in cis were searched and the regulatory network was formed. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the target genes were strongly associated with immune system, signaling molecular and interaction, endocrine system, and signal transduction. The differently expressed lncRNAs were selected and verified by quantitative real-time polymerase chain reaction (RT-qPCR). Our study, for the first time, analyzed a comprehensive lncRNA expression profile in DEFs following DTMUV infection. The analysis provided a view on the important roles of lncRNAs in gene regulation and DTMUV infection.