Nanostructured lipid system as a strategy to improve the anti-Candida albicans activity of Astronium sp.
Nanostructured lipid system as a strategy to improve the anti-Candida albicans activity of Astronium sp.
复制标题
DOI：
10.2147/ijn.s79684
复制
发表时间：
2015
影响因子：
8
通讯作者：
Bauab TM
中科院分区：
文献类型：
--
作者：
Bonifácio BV;Ramos MA;da Silva PB;Negri KM;de Oliveira Lopes É;de Souza LP;Vilegas W;Pavan FR;Chorilli M;Bauab TM
天竺葵属（天竺葵科）包括天竺葵、天竺葵和天竺葵等，它们具有抗炎、抗溃疡、愈合和抗菌的特性。纳米结构脂质系统能够增强植物提取物的作用，减少所需的剂量和副作用，提高抗菌活性。本研究旨在评价一种纳米结构脂质系统，该脂质系统被开发作为一种策略，以提高天竺树茎叶氢乙醇提取物的抗白色念珠菌活性。通过微稀释技术评估了体外对白色念珠菌（ATCC 18804）的抗真菌活性。在体外实验的基础上，利用外阴阴道假丝酵母菌感染模型进行了体内实验，结果表明，astroonium具有最佳的抗真菌活性和选择性指数。在这些实验中，提取物要么单独使用，要么加入到纳米结构脂质体系中（包括10%油相，10%表面活性剂和80%水相）。结果表明，在加入纳米结构体系之前，最低抑制浓度为125.00µg/mL；当该提取物掺入后的最低抑制浓度为15.62µg/mL时，该活性进一步增强。体内实验数据表明，纳米结构联合提取物的抗真菌效果优于非联合提取物和阳性对照（两性霉素B）。这些结果表明，该纳米结构脂质系统可用于提高体外和体内抗c抗体的策略。天竺葵水乙醇提取物的白念珠菌活性研究。
The genus Astronium (Anacardiaceae) includes species, such as Astronium fraxinifolium, Astronium graveolens, and Astronium urundeuva, which possess anti-inflammatory, anti-ulcerogenic, healing, and antimicrobial properties. Nanostructured lipid systems are able to potentiate the action of plant extracts, reducing the required dose and side effects and improving antimicrobial activity. This work aims to evaluate a nanostructured lipid system that was developed as a strategy to improve the anti-Candida albicans activity of hydroethanolic extracts of stems and leaves from Astronium sp. The antifungal activity against C. albicans (ATCC 18804) was evaluated in vitro by a microdilution technique. In addition to the in vitro assays, the Astronium sp. that showed the best antifungal activity and selectivity index was submitted to an in vivo assay using a model of vulvovaginal candidiasis infection. In these assays, the extracts were either used alone or were incorporated into the nanostructured lipid system (comprising 10% oil phase, 10% surfactant, and 80% aqueous phase). The results indicated a minimal inhibitory concentration of 125.00 µg/mL before incorporation into the nanostructured system; this activity was even more enhanced when this extract presented a minimal inhibitory concentration of 15.62 µg/mL after its incorporation. In vivo assay dates showed that the nanostructure-incorporated extract of A. urundeuva leaves was more effective than both the unincorporated extract and the antifungal positive control (amphotericin B). These results suggest that this nanostructured lipid system can be used in a strategy to improve the in vitro and in vivo anti-C. albicans activity of hydroethanolic extracts of Astronium sp.