Analysis methods of human cell migration
Analysis methods of human cell migration
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DOI：
10.1016/j.yexcr.2005.03.029
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发表时间：
2005-07-15
影响因子：
3.7
通讯作者：
Zaenker, KS
中科院分区：
文献类型：
--
作者：
Entschladen, F;Drell, TL;Zaenker, KS
特化细胞的自主迁移是成人有机体生理和病理功能的基本特征。因此，白细胞、成纤维细胞、干细胞以及肿瘤细胞成为广泛研究领域中深入研究的对象。因此，已经建立了多种方法来分析趋化性和趋化性细胞迁移，从易于操作的二维表面迁移测定到高度专业化的三维和活体分析方法。现在很明显，通过这些不同的迁移测定获得的结果有很大不同。因此，本综述概述了当前使用的迁移测定，描述了这些方法，并批判性地阐明了每种方法的特定优点和缺点。圆点 2005 Elsevier Inc. 保留所有权利。
The autonomous migration of specialized cells is an essential characteristic in both physiological and pathological functions in the adult human organism. Leukocytes, fibroblasts, and stem cells, but also tumor cells, are thus the subject of intense investigation in a broad range of research fields. A wide spectrum of methods have therefore been established to analyze chemokinetic and chemotactic cell migration, ranging from easy-to-handle two-dimensional surface migration assays to highly specialized three-dimensional and intravital analysis methods. It is now manifest that the results obtained with these various migration assays substantially differ. This review therefore gives an overview of the migration assays which are currently in use, describes the methods, and critically enlightens the particular advantages and disadvantages of each method. circle dot 2005 Elsevier Inc. All rights reserved.