UBIQUINONE BIOSYNTHESIS IN EUKARYOTIC CELLS - TISSUE DISTRIBUTION OF MESSENGER-RNA ENCODING 3,4-DIHYDROXY-5-POLYPRENYLBENZOATE METHYLTRANSFERASE IN THE RAT AND MAPPING OF THE COQ3 GENE TO MOUSE CHROMOSOME-4
UBIQUINONE BIOSYNTHESIS IN EUKARYOTIC CELLS - TISSUE DISTRIBUTION OF MESSENGER-RNA ENCODING 3,4-DIHYDROXY-5-POLYPRENYLBENZOATE METHYLTRANSFERASE IN THE RAT AND MAPPING OF THE COQ3 GENE TO MOUSE CHROMOSOME-4
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DOI：
10.1006/abbi.1994.1362
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发表时间：
1994-08-15
影响因子：
3.9
通讯作者：
CLARKE, CF
中科院分区：
文献类型：
--
作者：
MARBOIS, BN;XIA, YR;CLARKE, CF
最近报道了大鼠COQ 3基因的cDNA的分离和测序。COQ 3基因编码3，4-二羟基-5-聚异戊二烯基苯甲酸甲酯转移酶，一种辅酶和生物合成中的酶。在这项研究中，大鼠COQ 3 cDNA已被用来检查COQ 3基因的表达和染色体定位。在分析的每个组织中检测到COQ 3 mRNA，这与在大多数组织中从头合成解释了观察到的Q水平的想法一致。睾丸、心脏和骨骼肌中的含量最高。大鼠COQ 3 cDNA与来自多个物种的基因组DNA杂交，表明3，4-二羟基-5-聚异戊二烯苯甲酸酯甲基转移酶的保守性。COQ 3基因的单拷贝似乎存在于大鼠和小鼠中。通过分析种间回交中的限制性片段长度变体，将COQ 3基因定位于小鼠4号染色体上，在标记D4 Mit 4近端的3.7 +/-2.6cM的位置处。该基因的图谱位置，命名为Coq 3，将其放置在小鼠vacillans或vc突变附近。ve小鼠表现出的症状与人类报告的缺陷相似。(C)1994年出版社出版。
The isolation and sequence of a rat cDNA corresponding to the rat COQ3 gene was recently reported. The COQ3 gene encodes 3,4-dihydroxy-5-polyprenylbenzoate methyltransferase, an enzyme in coenzyme and biosynthesis. In this study, the rat COQ3 cDNA has been used to examine the expression and chromosomal localization of the COQ3 gene. COQ3 mRNA was detected in every tissue analyzed, consistent with the idea that in most tissues de novo synthesis accounts for the observed levels of Q. Highest levels were present in testis, heart, and skeletal muscle. The rat COQ3 cDNA hybridized to genomic DNA from multiple species, suggesting the conserved nature of the 3,4-dihydroxy-5-polyprenylbenzoate methyltransferase. A single copy of the COQ3 gene appears to be present in rats and mice. By analyzing a restriction fragment length variant in an interspecific backcross, the COQ3 gene was localized to mouse chromosome 4, at a position 3.7 +/- 2.6 cM proximal to the marker D4Mit4. The map position of the gene, designated Coq3, places it in close proximity to the mouse vacillans or vc mutation. The symptoms exhibited by the ve mice are similar to those reported for and deficiencies in humans. (C) 1994 Academic Press, Inc.