ARTIFICIAL FERTILIZATION OF GAMETES FROM THE SOUTH-AFRICAN CLAWED FROG, XENOPUS-LAEVIS
ARTIFICIAL FERTILIZATION OF GAMETES FROM THE SOUTH-AFRICAN CLAWED FROG, XENOPUS-LAEVIS
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DOI：
10.1002/mrd.1120030106
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
CORTON, GL
中科院分区：
文献类型：
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作者：
HOLLINGER, TG;CORTON, GL
在系统调控环境因子的基础上，建立了一种重复性好、效率高的非洲爪蟾卵受精方法。不同浓度的受精培养基中的各个成分的影响进行了测试，通过测量果冻肿胀，精子活力，和精子寿命。结果用于开发用于受精的改进培养基，其由41.25 mM NaCl、1.25 mM KCl、0.25 mM CaCl2、0.0625 mM MgCl2、0.5 mM Na2HPO4、2.5 mM HEPES、1.9 mM NaOH组成，最终pH（2°）7.8。
A reproducible and effective method for fertilization eggs of Xenopus laevis was developed based of systematic manipulation of environmental factors. The effects of varying concentrations of individual components of a fertilization medium were tested by measuring jelly swelling, sperm motility, and sperm longevity. Results were used to develop an improved medium for fertilization, consisting of 41.25 mM NaCl, 1.25 mM KCl, 0.25 mM CaCl2, 0.0625 mM MgCl2, 0.5 mM Na2HPO4, 2.5 mM HEPES, 1.9 mM NaOH, final pH(2°) 7.8.