Multispectral imaging of organ viability during uterine transplantation surgery in rabbits and sheep
Multispectral imaging of organ viability during uterine transplantation surgery in rabbits and sheep
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DOI：
10.1117/1.jbo.21.10.106006
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发表时间：
2016-10-01
影响因子：
3.5
通讯作者：
Elson, Daniel S.
中科院分区：
文献类型：
--
作者：
Clancy, Neil T.;Saso, Srdjan;Elson, Daniel S.
子宫移植手术(UTX)被认为是一种治疗先天性子宫缺失或手术切除子宫的永久性绝对型子宫因素不孕(AUFI)的方法。手术成功地附着器官及其相关的血管对器官的再灌注和长期存活是至关重要的。光谱成像技术已经证明了在医学应用中测量血流动力学的潜力。这包括通过获取不同波段的组织图像来测量反射光谱。然后，通过对光-组织相互作用的建模，可以从这些光谱中提取每个像素的组织成分的测量结果。在绵羊和兔UTX模型上使用多光谱成像(MSI)腹腔镜来研究手术后血氧饱和度的短期和长期变化。整个器官在供体和受体动物身上进行成像，同时通过脉搏血氧仪进行点数测量。成像结果证实，手术后移植器官重新建立了足够的血流灌注。MSI测量的眼角氧合趋势与脉搏血氧饱和度读数一致，显示吻合血管后立即血氧分压降低。长期结果显示血氧饱和度恢复到术前水平。(C)作者。由SPIE在知识共享署名3.0未移植许可下发布。分发或复制本作品的全部或部分内容需要提供原始出版物的全部归属，包括其DOI。
Uterine transplantation surgery (UTx) has been proposed as a treatment for permanent absolute uterine factor infertility (AUFI) in the case of the congenital absence or surgical removal of the uterus. Successful surgical attachment of the organ and its associated vasculature is essential for the organ's reperfusion and long-term viability. Spectral imaging techniques have demonstrated the potential for the measurement of hemodynamics in medical applications. These involve the measurement of reflectance spectra by acquiring images of the tissue in different wavebands. Measures of tissue constituents at each pixel can then be extracted from these spectra through modeling of the light-tissue interaction. A multispectral imaging (MSI) laparoscope was used in sheep and rabbit UTx models to study short-and long-term changes in oxygen saturation following surgery. The whole organ was imaged in the donor and recipient animals in parallel with point measurements from a pulse oximeter. Imaging results confirmed the re-establishment of adequate perfusion in the transplanted organ after surgery. Cornual oxygenation trends measured with MSI are consistent with pulse oximeter readings, showing decreased StO(2) immediately after anastomosis of the blood vessels. Long-term results show recovery of StO2 to preoperative levels. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.