INFLUENCE OF THE SEED POLYMER ON THE CHROMATOGRAPHIC PROPERTIES OF SIZE MONODISPERSE POLYMERIC SEPARATION MEDIA PREPARED BY A MULTISTEP SWELLING AND POLYMERIZATION METHOD
INFLUENCE OF THE SEED POLYMER ON THE CHROMATOGRAPHIC PROPERTIES OF SIZE MONODISPERSE POLYMERIC SEPARATION MEDIA PREPARED BY A MULTISTEP SWELLING AND POLYMERIZATION METHOD
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DOI：
10.1002/pola.1993.080310819
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发表时间：
1993-07-01
影响因子：
--
通讯作者：
FRECHET, JMJ
中科院分区：
文献类型：
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作者：
HOSOYA, K;FRECHET, JMJ
采用两对单体和两种致孔溶剂，通过多步溶胀聚合法制备了单分散聚合物颗粒基分离介质。它们的色谱性能进行了比较，通过相应的悬浮聚合方法制备的珠粒，而不使用种子聚合物，以确定种子聚合物对它们的多孔结构的影响。当由单体和致孔溶剂组成的溶胀颗粒含有至少一种用于聚苯乙烯种子聚合物的良溶剂，使其保留在聚合介质中时，观察到多孔结构的大变化。相比之下，当聚苯乙烯种子颗粒被排除在溶胀的油滴之外时，由于其在单体和致孔溶剂中的溶解性差，在多步溶胀和聚合方法与悬浮聚合方法之间的色谱性质如孔体积、孔径、孔径分布或保留选择性没有差异。由于多步溶胀和聚合方法与悬浮方法之间的唯一区别是使用种子聚合物，因此看起来在扩大的溶胀液滴中非常少量（<1%v/v）的种子聚合物作为致孔剂起重要作用，并影响单分散聚合物颗粒基分离介质的多孔结构以及色谱性质。
Monodisperse polymer particle-based separation media were prepared by a multi-step swelling and polymerization method with two pairs of monomers and two porogenic solvents. Their chromatographic properties were compared to those of beads prepared by a corresponding suspension polymerization method without the use of seed polymer to ascertain the influence of the seed polymer on their porous structures. A large change in porous structure was observed when the swollen particle consisting of monomers and porogenic solvents contained at least one good solvent for the polystyrene seed polymer, allowing it to remain in the polymerizing medium. In contrast, when the polystyrene seed particle was excluded from the swollen oil droplets, due to its poor solubility in the monomers and the porogenic solvents, there was no difference in the chromatographic properties such as pore volume, pore size, pore size distribution, or retention selectivity between the multi-step swelling and polymerization method and the suspension polymerization method. Since the only difference between the multi-step swelling and polymerization method and the suspension method is the use of the seed polymer, it appears that a very small amount (< 1 % v/v) of seed polymers in the enlarged swollen droplets plays an important role as a porogen and affects the porous structure as well as the chromatographic properties of the mono-disperse polymer particle-based separation media.