Optical tweezers for measuring red blood cell elasticity: application to the study of drug response in sickle cell disease
Optical tweezers for measuring red blood cell elasticity: application to the study of drug response in sickle cell disease
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DOI：
10.1034/j.1600-0609.2003.00027.x
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发表时间：
2003-04-01
影响因子：
3.1
通讯作者：
Saad, STO
中科院分区：
文献类型：
--
作者：
Brandao, MM;Fontes, A;Saad, STO
红细胞的变形能力是微循环中血流的关键决定因素。通过用光镊捕获红细胞 (RBC) 并将它们拖过粘性液体，我们能够测量它们的整体弹性。我们测量并比较了 15 名纯合患者 (HbSS) 的红细胞变形能力，其中包括 5 名服用羟基脲 (HU) 至少 6 个月的患者 (HbSS/HU)、10 名镰状细胞性状 (HbAS) 受试者和 35 名正常对照。我们的结果表明，血红蛋白 S (HbS) 受试者（HbSS 和 HbAS）的红细胞变形能力显着较低，但 HbSS/HU 细胞除外，其变形能力与正常对照相似。我们的数据表明，激光光镊技术能够检测服用 HU 的受试者的 HbS RBC 差异，并将 RBC 与正常对照和 HbAS 区分开来，表明这是一种非常灵敏的方法，可用于检测镰状细胞病的药物反应。
The deformability of erythrocytes is a critical determinant of blood flow in microcirculation. By capturing red blood cells (RBC) with optical tweezers and dragging them through a viscous fluid we were able to measure their overall elasticity. We measured, and compared, the RBC deformability of 15 homozygous patients (HbSS) including five patients taking hydroxyurea (HU) for at least 6 months (HbSS/HU), 10 subjects with sickle cell trait (HbAS) and 35 normal controls. Our results showed that the RBC deformability was significantly lower in haemoglobin S (HbS) subjects (HbSS and HbAS), except for HbSS/HU cells, whose deformability was similar to the normal controls. Our data showed that the laser optical tweezers technique is able to detect differences in HbS RBC from subjects taking HU, and to differentiate RBC from normal controls and HbAS, indicating that this is a very sensitive method and can be applied for detection of drug-response in sickle cell disease.