The molecular mechanism underlying cadmium resistance in NHX1 transgenicLemna turoniferawas studied by comparative transcriptome analysis
The molecular mechanism underlying cadmium resistance in NHX1 transgenicLemna turoniferawas studied by comparative transcriptome analysis
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通过比较转录组分析研究NHX1转基因青萍抗镉的分子机制
DOI：
10.1007/s11240-020-01909-z
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发表时间：
2020-08-12
影响因子：
3
通讯作者：
Wang，Yong
中科院分区：
文献类型：
--
作者：
Yao，Jie;Sun，Jinge;Wang，Yong
镉（Cd）是毒性最强的重金属之一，对环境构成严重威胁。我们前期的研究表明，过表达Na+/H+Antiporter（AtNHX1）基因的转基因青萍（以下简称转基因）比野生型植物（WT）更能耐受Cd2+胁迫。为了进一步探讨镉耐药性的分子机制，进行了全面的转录组分析和比较。对于 RNA-Seq，转基因和 WT 用 50 μM CdCl2 处理 24 小时。转基因样本和野生型样本之间总共发现了 2247 个差异表达的单基因 (DEG)，其中包括 1195 个上调基因和 1052 个下调基因。与不定根（AR）、侧根（LR）和根毛（RH）起始相关的基因下调可能阻止了根的脱落。钙信号传导和 ROS 积累受到镉胁迫的调节。此外，参与海藻糖和R-S谷胱甘肽生产的基因的激活增强了浮萍的镉抗性。下调参与 Cd2+ 转运的基因和上调参与液泡隔离的基因可能导致 Transgenic 相对于 WT 的耐受性增加。这些发现可能为提高植物镉抗性和植物修复提供新思路。
Cadmium (Cd) is one of the most toxic heavy metals, which poses a serious threat to the environment. Our previous studies revealed that transgenicLemna turonifera(hereafter mentioned as Transgenic) over expressing Na+/H+Antiporter (AtNHX1) gene was more tolerant to Cd2+stress than the Wild type plants (WT). To further explore the molecular mechanism underlying cadmium resistance, comprehensive transcriptome analysis and comparison were performed. For RNA-Seq, the trannsgenic and WT were treated with 50 μM CdCl2for 24 h. In total, 2247 differentially expressed unigenes (DEGs) including 1195 upregulated and 1052 downregulated ones were discovered between Transgenic and WT samples. The downregulated genes associated with the adventitious root (AR), lateral root (LR) and root hair (RH) initiation might have prevented the root from abscission. Calcium signaling and ROS accumulation were regulated in response to cadmium stress. Moreover, the activation of genes involved in the production of trehalose and R-S glutathione increased the cadmium resistance of duckweed. Downregulation of genes involved in the transport of Cd2+and upregulation of genes involved in vacuolar sequestration might have resulted in increased tolerance of Transgenic over WT. These findings might provide new ideas for improving plant cadmium resistance and phytoremediation.