ENHANCED INTERFERON PRODUCTION FROM CHICK EMBRYO CELLS AGED IN IN VITRO
ENHANCED INTERFERON PRODUCTION FROM CHICK EMBRYO CELLS AGED IN IN VITRO
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DOI：
10.1016/0042-6822(67)90274-7
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发表时间：
1967-01-01
期刊：
影响因子：
3.7
通讯作者：
MARCUS, PI
中科院分区：
文献类型：
--
作者：
CARVER, DH;MARCUS, PI
在体外老化的鸡胚细胞单层上进行试验时，辛德毕斯病毒的空斑形成效率降低了1000倍，空斑大小明显减小。对纽卡斯尔病毒的空斑形成率和空斑大小无影响。当细胞单层在以下条件下建立时，与体外老化相关的导致空斑形成效率降低的反应会显著减缓：在B-H培养基而不是标准生长培养基中建立;保持在31 ℃;给予每日更换培养基的方案;或胰蛋白酶化并用作年轻的二次培养物。每细胞病毒平均产量的损失伴随着辛德毕斯病毒在老化单层上的噬斑效率的损失。在感染时向老化细胞中加入放线菌素D完全消除了辛德毕斯病毒复制的抑制。发现在体外老化7天的细胞比1-2天龄的细胞产生高达32倍的干扰素，并且对干扰素的作用更敏感。辛德毕斯病毒空斑形成效率和老化细胞产量的降低是由于它们在适当刺激下合成干扰素的能力增强所致。接触抑制的过程及其伴随的大分子合成的调节似乎与衰老现象有关，因为它可能在细胞中产生“增强的去抑制性”的普遍状态。
The plaque-forming efficiency of Sindbis virus decreased as much as 1000-fold, and plaque size was diminished markedly, when tested on chick embryo cell monolayers aged in vitro. The plaquing efficiency and plaque size of Newcastle disease virus was unaffected. The reaction(s) associated with aging in vitro which lead to lowered plaquing efficiency are slowed considerably when cell monolayers are: established in Simpson-Hirst medium rather than standard growth medium; held at 31 degrees; given a regimen of daily medium changes; or trypsinized and used as young secondary cultures. A loss in the average yield of virus per cell accompanies the loss in plaquing efficiency of Sindbis virus on aged monolayers. Adding actinomycin D to the aged cells at the time of infection eliminated completely the inhibition of Sindbis virus replication. Cells aged for 7 days in vitro were found to produce up to 32 times more interferon than cells 1-2 days old and were more sensitive to the action of interferon. The decrease in efficiency of Sindbis virus plaquing and yield in aged cells is accounted for by their development of an enhanced capacity to synthesize interferon upon appropriate stimulation. The process of contact inhibition and its concomitant regulation of macro-molecular synthesis seems implicated in the aging phenomenon in that it may produce a generalized state of "enhanced derepressibility" in the cell.