PLASTOCHRON3/GOLIATH encodes a glutamate carboxypeptidase required for proper development in rice
PLASTOCHRON3/GOLIATH encodes a glutamate carboxypeptidase required for proper development in rice
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DOI：
10.1111/j.1365-313x.2009.03841.x
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发表时间：
2009-06-01
期刊：
影响因子：
7.2
通讯作者：
Nagato, Yasuo
中科院分区：
文献类型：
--
作者：
Kawakatsu, Taiji;Taramino, Graziana;Nagato, Yasuo
植物体的大部分地上部分是茎尖分生组织（SAM）持续活动的产物。叶片是地上植物体的主要组成部分，其时空分布主要决定着地上植物的枝状构型。在这里，我们报道了水稻基因PLASTOCHRON3 (PLA3)/GOLIATH （GO）的鉴定，该基因调节各种发育过程，包括叶片起始率（plasochron）。PLA3/GO编码谷氨酸羧肽酶，该酶被认为能分解酸性小肽并产生小信号分子。Pla3表现出与pla1和pla2相似的表型——生育期缩短，叶片早熟成熟和轴枝枝到茎的转化。但与pla1和pla2相比，pla3表现出胚增大、种子胎生、SAM维持缺陷和叶片形态异常等多效性表型。与这些多效表型一致，PLA3在整个植物体内表达，并参与植物激素稳态。双突变分析表明，PLA1、PLA2和PLA3是独立调控的，但功能冗余。我们的研究结果表明，PLA3调节与许多发育过程相关的各种信号通路。
P>Most aerial parts of the plant body are products of the continuous activity of the shoot apical meristem (SAM). Leaves are the major component of the aerial plant body, and their temporal and spatial distribution mainly determines shoot architecture. Here we report the identification of the rice gene PLASTOCHRON3 (PLA3)/GOLIATH (GO) that regulates various developmental processes including the rate of leaf initiation (the plastochron). PLA3/GO encodes a glutamate carboxypeptidase, which is thought to catabolize small acidic peptides and produce small signaling molecules. pla3 exhibits similar phenotypes to pla1 and pla2- a shortened plastochron, precocious leaf maturation and rachis branch-to-shoot conversion in the reproductive phase. However, in contrast to pla1 and pla2, pla3 showed pleiotropic phenotypes including enlarged embryo, seed vivipary, defects in SAM maintenance and aberrant leaf morphology. Consistent with these pleiotropic phenotypes, PLA3 is expressed in the whole plant body, and is involved in plant hormone homeostasis. Double mutant analysis revealed that PLA1, PLA2 and PLA3 are regulated independently but function redundantly. Our results suggest that PLA3 modulates various signaling pathways associated with a number of developmental processes.