The cellular niche of Listeria monocytogenes infection changes rapidly in the spleen
The cellular niche of Listeria monocytogenes infection changes rapidly in the spleen
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DOI：
10.1002/eji.200838718
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发表时间：
2009-02-01
影响因子：
5.4
通讯作者：
Miller, Mark J.
中科院分区：
文献类型：
--
作者：
Aoshi, Taiki;Carrero, Javier A.;Miller, Mark J.
脾脏是宿主对全身细菌感染作出反应的重要器官。许多细胞类型和细胞表面受体已被证明在细菌的捕获和控制中发挥作用，但这些通常是单独研究的，并且各种吞噬细胞如何协同控制细菌感染的连贯图像尚未出现。我们分析了单核增生李斯特菌（Listeria monocytogenes， LM）在感染早期的原位细胞分布。使用免疫组织化学方法，五种不同的吞噬细胞群在静脉注射后含有LM，并大致占组织切片中所有细菌信号。我们的分析表明，LM最初在边缘区被广泛的吞噬细胞捕获，在那里LM的生长似乎受到控制。LM在吞噬细胞群内的细胞分布在最初几个小时内迅速变化，在边缘区巨噬细胞中减少，在CD11c DC中短暂增加。4-6小时后，淋巴结转移到小动脉周围淋巴鞘，形成感染灶，淋巴结呈指数增长。
The spleen is an important organ for the host response to systemic bacterial infections. Many cell types and cell surface receptors have been shown to play role in the capture and control of bacteria, yet these are often studied individually and a coherent picture has yet to emerge of how various phagocytes collaborate to control bacterial infection. We analyzed the cellular distribution of Listeria monocytogenes (LM) in situ during the early phase of infection. Using an immunohistochemistry approach, five distinct phagocyte populations contained LM after i.v. challenge and accounted for roughly all bacterial signal in tissue sections. Our analysis showed that LM was initially captured by a wide range of phagocytes in the marginal zone, where the growth of LM appeared to be controlled. The cellular distribution of LM within phagocyte populations changed rapidly during the first few hours, decreasing in marginal zone macrophages and transiently increasing in CD11c DC. After 4-6 h LM was transported to the periarteriolar lymphoid sheath where the infective foci developed and LM grew exponentially.