Modulation of NB4 promyelocytic leukemic cell machinery by Anaplasma phagocytophilum
Modulation of NB4 promyelocytic leukemic cell machinery by Anaplasma phagocytophilum
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DOI：
10.1016/j.ygeno.2005.05.008
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发表时间：
2005-09-01
期刊：
影响因子：
4.4
通讯作者：
Fikrig, E
中科院分区：
文献类型：
--
作者：
Pedra, JHF;Sukumaran, B;Fikrig, E
嗜吞噬细胞无形体是一种革兰氏阴性专性细胞内细菌，存在于中性粒细胞内。我们评估了A.利用高密度寡核苷酸芯片、双向差示凝胶电泳和液相色谱-质谱联用技术检测嗜吞噬evtophilum在NB 4早幼粒细胞白血病细胞中的感染。我们的数据显示，A。嗜吞噬细胞菌改变了转录因子、细胞粘附分子、信号转导基因和促炎细胞因子的表达。然而，Toll样受体、MYD 88、RNF 36、IRF 3和TBK 1以及NF-κ B基因抑制剂的表达没有改变。A.嗜吞噬细胞菌感染也改变了NB 4细胞的凋亡程序，并导致抗凋亡基因（MCL 1和BFL 1）的转录增加。铁代谢基因（轻多肽铁蛋白链、转铁蛋白和转铁蛋白受体）的转录和翻译发生了显著改变，表明A.嗜吞噬细胞菌感染和铁代谢。我们的研究清楚地表明了A.嗜吞噬细胞菌感染对NB 4早幼粒细胞白血病细胞机制的影响。(c)2005年爱思唯尔公司All rights reserved.
Anaplasma phagocytophilum is a gram-negative obligate intracellular bacterium that persists within neutropbils. We assessed the impact of A. phagoevtophilum infection in NB4 promyelocytic leukemic cells using high-density oligoarray, two-dimensional differential gel electrophoresis and liquid chromatography-mass spectrometry. Our Affymetrix data revealed that A. phagocytophilum altered the expression of transcription factors, cell adhesion molecules, signal transduction genes, and proinflammatory cytokines. However, the expression of Toll-like receptors, MYD88, RNF36, IRF3, and TBK1 and inhibitors of the NF-kappa B gene was not altered. A. phagocytophilum infection also altered the apoptotic program of NB4 cells and resulted in increased transcription of antiapoptotic genes (MCL1 and BFL1). The transcription and translation of iron-metabolism genes (light polypeptide ferritin chain, transferrin, and the transferrin receptor) were significantly altered, suggesting a possible link between A. phagocytophilum infection and iron metabolism. Our study clearly demonstrates multifactorial effects of A. phagocytophilum infection on NB4 promyelocytic leukemic cell machinery. (c) 2005 Elsevier Inc. All rights reserved.