Rapid and Reliable Method for Determining Irradiation Histories of Ground Beef and Prawns by Measuring 5,6-Dihydrothymidine
Rapid and Reliable Method for Determining Irradiation Histories of Ground Beef and Prawns by Measuring 5,6-Dihydrothymidine
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通过测量 5,6-二氢胸苷快速可靠地测定碎牛肉和虾的辐照历史的方法
DOI：
10.1021/acs.jafc.7b03266
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发表时间：
2017
影响因子：
6.1
通讯作者：
Hirotaka Obana
中科院分区：
文献类型：
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作者：
Naoki Fukui;Satoshi Takatori;Yoko Kitagawa;Takuya Fujiwara;Etsuko Ishikawa;Takatomo Fujiyama;Keiji Kajimura;Masakazu Furuta;Hirotaka Obana
在建立5，6-二氢胸苷（DHdThd）液相色谱-串联质谱（LC-MS/MS）检测牛肝辐照历史方法的基础上，建立了一种快速、可靠的牛肉和对虾辐照历史检测方法。方法的改进包括：（1）在RNA酶步骤之前在DNA提取步骤中进行50%乙醇沉淀，（2）蛇毒磷酸二酯酶I用于DNA消化以促进DHdThd的释放，和（3）基质匹配的校准曲线用于通过LC-MS/MS分析测定DHdThd。该方法成功地测定了牛肉和对虾的辐照历史。此外，DHdThd和2-alkylcyclobutanones，这是一个既定的指数的照射历史的形成之间的密切相关性，观察碎牛肉。DNA中的DHdThd可能是一个有前途的候选人，为各种食物的辐照历史的新指标。
A rapid and reliable method for determining irradiation histories of ground beef and prawns was developed on the basis of a method for determining the irradiation history of beef liver by liquid chromatography–tandem mass spectrometry (LC-MS/MS) of 5,6-dihydrothymidine (DHdThd). Improvements in the method included the following: (1) 50% ethanol precipitation in the DNA extraction step was conducted before the RNase step, (2) snake venom phosphodiesterase I was used for DNA digestion to boost liberation of DHdThd, and (3) a matrix-matched calibration curve was used for determining DHdThd by LC-MS/MS analysis. This method successfully determined irradiation histories of ground beef and prawns. Furthermore, a close correlation between the formation of DHdThd and 2-alkylcyclobutanones, which are an established index of irradiation histories, was observed in ground beef. DHdThd in DNA could be a promising candidate for a new index of irradiation histories of various foods.