A conserved neuropeptide system links head and body motor circuits to enable adaptive behavior.
A conserved neuropeptide system links head and body motor circuits to enable adaptive behavior.
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DOI：
10.7554/elife.71747
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发表时间：
2021-11-12
期刊：
影响因子：
7.7
通讯作者：
Francis MM
中科院分区：
文献类型：
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作者：
Ramachandran S;Banerjee N;Bhattacharya R;Lemons ML;Florman J;Lambert CM;Touroutine D;Alexander K;Schoofs L;Alkema MJ;Beets I;Francis MM
神经调节剂促进适应行为，这些行为通常是复杂的，涉及在通常没有物理连接的电路中协调一致的活动变化。神经调节系统如何完成这些任务还不是很清楚。在这里，我们发现秀丽隐杆线虫NLP-12神经肽系统通过替代g蛋白偶联受体（GPCR）靶点CKR-1和CKR-2调节头部和体壁运动神经元的活性，从而形成对食物供应的反应。我们发现，在基础条件下，ckr-2缺失会减少运动时的身体弯曲深度。我们证明了CKR-1是一个功能性NLP-12受体，并确定了其在神经系统中的表达。与基础运动相反，头部运动神经元的偏向性CKR-1 GPCR刺激促进局部搜索过程中的转向。ckr-1的缺失降低了头部神经元的活性，减少了转向，而特异性的ckr-1过表达或头部神经元的激活促进了转向。因此，我们的研究表明，运动对食物供应变化的反应是通过头部或体壁运动回路的条件NLP-12刺激来调节的。
Neuromodulators promote adaptive behaviors that are often complex and involve concerted activity changes across circuits that are often not physically connected. It is not well understood how neuromodulatory systems accomplish these tasks. Here, we show that the Caenorhabditis elegans NLP-12 neuropeptide system shapes responses to food availability by modulating the activity of head and body wall motor neurons through alternate G-protein coupled receptor (GPCR) targets, CKR-1 and CKR-2. We show ckr-2 deletion reduces body bend depth during movement under basal conditions. We demonstrate CKR-1 is a functional NLP-12 receptor and define its expression in the nervous system. In contrast to basal locomotion, biased CKR-1 GPCR stimulation of head motor neurons promotes turning during local searching. Deletion of ckr-1 reduces head neuron activity and diminishes turning while specific ckr-1 overexpression or head neuron activation promote turning. Thus, our studies suggest locomotor responses to changing food availability are regulated through conditional NLP-12 stimulation of head or body wall motor circuits.