Expression of alpha and beta parvalbumin is differentially regulated in the rat organ of corti during development.
Expression of alpha and beta parvalbumin is differentially regulated in the rat organ of corti during development.
复制标题
在发育过程中，大鼠皮质器官中α和β小白蛋白的表达受到差异性调节。
DOI：
10.1002/neu.10289
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Simmons,DD
中科院分区：
文献类型：
--
作者：
Yang,D;Thalmann,I;Thalmann,R;Simmons,DD
小清蛋白 (PV) 家族的两种钙结合蛋白，即 α 同工型 (αPV) 和被称为癌调节蛋白 (OM) 的 β 同工型，在出生后发育过程中的大鼠耳蜗中进行了研究，并与胆碱能传出神经支配相关。通过RT-PCR分析，我们发现OM表达在出生后第2天（P2）和P4之间开始，并早在P10时达到峰值，而αPV mRNA在出生前开始表达，并在成年期保持高表达。原位杂交和免疫反应证实 OM 由大鼠耳蜗中的外毛细胞 (OHC) 独特表达，并在 P2 和 P4 之间沿耳蜗螺旋的传出神经支配后发生。与 OM 表达相反，αPV 免疫反应性在出生时在内毛细胞 (IHC) 和 OHC 中表达。橄榄耳蜗传出神经支配后，OHC 从 P5 开始表现出较弱的 OM 免疫反应性，并在 P10 后减弱 αPV 免疫反应性。在 OHC 传出神经支配之前分离的器官培养物中，OM 免疫反应性未能在 OHC 中形成，但 αPV 免疫反应性在 IHC 和 OHC 中仍然存在。相反，OHC 传出神经支配后分离的器官培养物显示 OHC 具有低水平的 OM 免疫反应性和高水平的 αPV 免疫反应性。这项研究表明，在耳蜗发育过程中，OM 和 αPV 在 OHC 中受到不同的调节。我们的研究结果进一步提出了 OHC 中 PV 蛋白的表达可能受到传出神经支配影响的可能性。 © 2003 Wiley periodicals, Inc. J Neurobiol 58: 479–492, 2004
The expression of two calcium‐binding proteins of the parvalbumin (PV) family, the α isoform (αPV) and the β isoform known as oncomodulin (OM), was investigated in the rat cochlea during postnatal development and related to cholinergic efferent innervation. Using RT‐PCR analysis, we found that OM expression begins between postnatal day 2 (P2) and P4, and peaks as early as P10, while αPV mRNA begins expression before birth and remains highly expressed into the adult period. Bothin situhybridization and immunoreactivity confirm that OM is uniquely expressed by the outer hair cells (OHCs) in the rat cochlea and occurs after efferent innervation along the cochlear spiral between P2 and P4. In contrast to OM expression, αPV immunoreactivity is expressed in both inner hair cells (IHCs) and OHCs at birth. Following olivocochlear efferent innervation, OHCs demonstrate weak OM immunoreactivity beginning at P5 and diminished αPV immunoreactivity after P10. In organ cultures isolated prior to the efferent innervation of OHCs, OM immunoreactivity failed to develop in OHCs, but αPV immunoreactivity remained present in both IHCs and OHCs. In contrast, organ cultures isolated after efferent innervation of OHCs show OHCs with low levels of OM immunoreactivity and high levels of αPV immunoreactivity. This study suggests that OM and αPV are differentially regulated in OHCs during cochlear development. Our findings further raise the possibility that the expression of PV proteins in OHCs may be influenced by efferent innervation. © 2003 Wiley Periodicals, Inc. J Neurobiol 58: 479–492, 2004