Thyroid transcription factor 1 and cytokeratins 1, 5, 10, 14 (34βE12) expression in basaloid and large-cell neuroendocrine carcinomas of the lung
Thyroid transcription factor 1 and cytokeratins 1, 5, 10, 14 (34βE12) expression in basaloid and large-cell neuroendocrine carcinomas of the lung
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DOI：
10.1053/hupa.2001.27110
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发表时间：
2001-09-01
期刊：
影响因子：
3.3
通讯作者：
Brambilla, E
中科院分区：
文献类型：
--
作者：
Sturm, N;Lantuéjoul, S;Brambilla, E
基底细胞样癌（BQ）和大细胞神经内分泌癌（LCNEC）是最近认识到的两种大细胞肺癌的变体，它们在形态上可能重叠，并通过LCNEC中神经内分泌标志物的表达来区分。由于甲状腺转录因子I（TTF-1）在肺腺癌中表达，但在鳞状细胞癌（SCC）中不表达，并且34 β E12识别一组基底干细胞特有的高分子量细胞角蛋白，我们假设这两种标记物有助于区分BC和LCNEC。TTF-1的免疫组化在40.9%的纯LCNEC中检测到，但在SCC的BC或基底细胞样变体中没有检测到。与此相反，34 β E12的免疫反应显示在所有的BC和基底细胞的SCC的变体，但只有1 LCNEC。布因固定在两种标记物的免疫检测中的效率低于福尔马林，因为其众所周知的对抗原保存的有害作用。TTF-1对LCNEC的特异性（100%）和34 β E12对BC的特异性（98.3%）均超过NE标记物。这些数据表明，TTF-1和34 β E12，与特定的神经内分泌标志物，代表了一组有用的抗体，在区分肿瘤表现为实性模式，栅栏，或假卵巢癌，TTF-1的表达排除BC的诊断，和34 β E12染色排除纯LCNEC。Copyright（C）2001 by W.B.桑德斯公司
Basaloid carcinoma (BQ and large-cell neuroendocrine carcinoma (LCNEC) are 2 recently recognized variants of large-cell lung carcinomas that may overlap in their morphology, and are discriminated by expression of neuroendocrine markers in LCNEC. Because thyroid transcription factor I (TTF-1) is expressed in lung adenocarcinomas but not in squamous cell carcinomas (SCC), and 34 beta E12 recognizes a set of high-molecular-weight cytokeratins characteristic of basal stem cells, we hypothesized that these 2 markers could help in distinguishing BC from LCNEC. Immunostaining for TTF-1 was detected in 40.9% of pure LCNEC but in no BC or basaloid variant of SCC. In contrast, immunoreactivity for 34 beta E12 was shown in all BC and basaloid variant of SCC but in only 1 LCNEC. Bouin fixation was less efficient than formalin in the immunodetection of both markers for its well-known deleterious effect on antigen preservation. Specificity of TTF-1 for LCNEC (100%) and that of 34 beta E12 for BC (98.3%) exceeded that of NE markers for distinction of these 2 entities. These data show that TTF-1 and 34 beta E12, in association with specific neuroendocrine markers, represent a useful panel of antibodies in differentiating carcinomas presenting with a solid pattern, palisading, or pseudorosettes, the expression of TTF-1 excluding the diagnosis of BC, and staining with 34 beta E12 excluding pure LCNEC. Copyright (C) 2001 by W.B. Saunders company.