Histopathology of normal skin and melanomas after nanosecond pulsed electric field treatment.
Histopathology of normal skin and melanomas after nanosecond pulsed electric field treatment.
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纳秒脉冲电场治疗后正常皮肤和黑色素瘤的组织病理学
DOI：
10.1097/cmr.0b013e32832f1558
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发表时间：
2009-12
影响因子：
2.2
通讯作者：
Schoenbach KH
中科院分区：
文献类型：
--
作者：
Chen X;James Swanson R;Kolb JF;Nuccitelli R;Schoenbach KH
纳秒脉冲电场(NsPEF)可以影响体外培养的细胞内结构。本研究显示了nsPEFS对SKH-1小鼠正常皮肤和B16-F10黑色素瘤的肿瘤生长、肿瘤体积和组织学特征的直接影响。给雌性SKH-1小鼠注射B16-F10建立黑色素瘤模型。100次脉冲nsPEF(场强40kV/cm；持续时间300 ns；上升时间30 ns；重复频率2 Hz)治疗后，采用透射法、苏木精-伊红染色光镜和透射电子显微镜观察肿瘤生长和组织学变化。黑色素瘤内的黑色素和铁也用特殊的染色进行了检测。NsPEF治疗后，肿瘤生长受到抑制，与对照组相比，肿瘤体积缩小(P<；0.05)。NsPEF治疗组肿瘤体积较对照组明显缩小(P<；0.01)。苏木精-伊红染色和透射电子显微镜显示肿瘤的形态改变和核萎缩。Fontana-Masson染色显示nsPEF可外化黑色素。铁染色显示nsPEF在处理的组织中引起轻微出血。组织学证实，重复应用nsPEF扰乱了血管网络。NsPEF治疗可以显著破坏血管系统，减少皮下黑色素瘤的发展，并产生肿瘤细胞收缩和核收缩，同时但不是永久性地损害治疗区周围的健康皮肤组织，我们将其归因于针电极周围的高度局部化电场。
Nanosecond pulsed electric fields (nsPEFs) can affect the intracellular structures of cells in vitro. This study shows the direct effects of nsPEFs on tumor growth, tumor volume, and histological characteristics of normal skin and B16-F10 melanoma in SKH-1 mice. A melanoma model was set up by injecting B16-F10 into female SKH-1 mice. After a 100-pulse treatment with an nsPEF (40-kV/cm field strength; 300-ns duration; 30-ns rise time; 2-Hz repetition rate), tumor growth and histology were studied using transillumination, light microscopy with hematoxylin and eosin stain and transmission electron microscopy. Melanin and iron within the melanoma tumor were also detected with specific stains. After nsPEF treatment, tumor development was inhibited with decreased volumes post-nsPEF treatment compared with control tumors (P<0.05). The nsPEF-treated tumor volume was reduced significantly compared with the control group (P<0.01). Hematoxylin and eosin stain and transmission electron microscopy showed morphological changes and nuclear shrinkage in the tumor. Fontana–Masson stain indicates that nsPEF can externalize the melanin. Iron stain suggested nsPEF caused slight hemorrhage in the treated tissue. Histology confirmed that repeated applications of nsPEF disrupted the vascular network. nsPEF treatment can significantly disrupt the vasculature, reduce subcutaneous murine melanoma development, and produce tumor cell contraction and nuclear shrinkage while concurrently, but not permanently, damaging peripheral healthy skin tissue in the treated area, which we attribute to the highly localized electric fields surrounding the needle electrodes.