Annual fish as a genetic model for aging.
Annual fish as a genetic model for aging.
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DOI：
10.1093/gerona/59.2.b101
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发表时间：
2004-02
期刊：
影响因子：
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通讯作者：
Michael B. Herrera;P. Jagadeeswaran
中科院分区：
文献类型：
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作者：
Michael B. Herrera;P. Jagadeeswaran
衰老遗传学和长寿基因鉴定的进展在很大程度上归功于秀丽隐杆线虫和黑腹果蝇等模式生物。然而，从这些无脊椎动物身上获得的知识将无法识别脊椎动物特有的长寿基因。老鼠的寿命相对较长，约为3年，这限制了它在长寿基因筛选中的应用。鱼类已被用于衰老研究。然而，缺乏对鱼类生存曲线的系统比较。在本研究中，我们比较了斑马鱼和2种不同的一年生鱼类，即黑颈白颈鱼（Cynolebias nigripinnis）和棘鱼（Nothobranchius rachovii）的生存曲线。这些研究证实了诺霍氏branchus的寿命最短（8.5个月，这段时间有10%的种群存活）。我们还证实了在实验室条件下繁殖拉氏诺thobranchus rachovii是可能的，并且表明它们的胚胎可以保存几个月，并且通过加水可以随时孵化。此外，我们从公共数据库中获得的斑马鱼和河豚的同源基因组序列的71个扩增片段中分离出31个cDNA标记，表明rhovii nothobranchus约有40%的基因可以很容易地分离出来。因此，在实验室条件下繁殖的能力和cDNA标记的可用性，以及相对较短的寿命的主要优势，使棘鳃棘鱼成为一种有吸引力的脊椎动物遗传模型，而不是其他可用的脊椎动物模型。
Advancement in the genetics of aging and identification of longevity genes has been largely due to the model organisms such as Caenorhabditis elegans and Drosophila melanogaster. However, knowledge gained from these invertebrates will not be able to identify vertebrate-specific longevity genes. The mouse has a relatively long life span of about 3 years, which limits its utility for screening of longevity genes. Fish have been used in aging studies. However, systematic comparison of survivorship curves for fish is lacking. In this study, we compared the survivorship curves of zebrafish and 2 different annual fish, namely, Cynolebias nigripinnis and Nothobranchius rachovii. These studies established that Nothobranchius rachovii has the shortest life span (8.5 months, at which time 10% of population remains). We also established that it is possible to breed Nothobranchius rachovii under laboratory conditions, and showed that their embryos can be stored for several months and hatched at any time by adding water. In addition, we have isolated 31 cDNA markers out of 71 attempted amplifications based on corresponding homologous genomic sequences in zebrafish and Fugu available from public databases, suggesting that approximately 40% of the genes from Nothobranchius rachovii could be easily isolated. Thus, the ability to be bred under laboratory conditions and the availability of cDNA markers for mapping, along with the major advantage of a relatively short life span, make Nothobranchius rachovii an attractive vertebrate genetic model for aging over other available vertebrate models.