NEGATIVE STAINING OF PHOSPHOLIPIDS + THEIR STRUCTURAL MODIFICATION BY-SURFACE ACTIVE AGENTS AS OBSERVED IN ELECTRON MICROSCOPE
NEGATIVE STAINING OF PHOSPHOLIPIDS + THEIR STRUCTURAL MODIFICATION BY-SURFACE ACTIVE AGENTS AS OBSERVED IN ELECTRON MICROSCOPE
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DOI：
10.1016/s0022-2836(64)80115-7
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发表时间：
1964-01-01
影响因子：
5.6
通讯作者：
HORNE, RW
中科院分区：
文献类型：
--
作者：
BANGHAM, AD;HORNE, RW
应用阴性染色技术在电子显微镜下研究脂质相结构。卵磷脂分散体含有由同心片层组成的球粒。脂质层宽度估计为44 A。杆状元素也被观察到，并被解释为与薄片长轴成直角排列的脂质分子群。等摩尔比的卵磷脂-胆固醇制剂被描述为与单独卵磷脂基本相同。皂素修饰了卵磷脂-胆固醇的层状结构，从而产生一系列中心间距为120a，直径为85a的穿孔。描述了分离的环和螺旋结构，估计直径为220 A，轴向孔为68 A。螺旋结构沿轴具有明显的58 ~ 59 a的周期性。卵磷脂分散体在水中与溶卵磷脂混合后逐渐被破坏。片层的串珠是一个明显的特征。卵磷脂和黄体酮在水中的分散产生了约20至22 a的线结构。
The negative-staining technique was applied to the study of lipid phase structures in the electron micro-scope. Lecithin dispersions were seen to contain spherulites composed of concentric lamellae. The width of the lipid layer was estimated to be 44 A. Rod-shaped elements were also observed and interpreted as being groups of lipid molecules arranged at right angles to the long axis of the lamellae. Preparations of lecithin-cholesterol of equal molar proportions were described as being basically the same as lecithin alone. Saponin modified the lamellar structure of lecithin-cholesterol in such a way as to produce an array of perforations with a centre-to-centre spacing of 120 A and with a diameter of 85 A. Separated rings together with helical structures estimated to be 220 A in diameter with an axial hole of 68 A were described. The helical structures showed a well-defined periodicity of 58 to 59 A along the axis. Lecithin dispersions were shown to be progressively disrupted after mixing with lysolecithin in water. Beading of the lamellae was a pronounced feature. Lecithin progesterone dispersions in water produced a line structure of about 20 to 22 A across.