Transgenic Plants That Express the Phytoplasma Effector SAP11 Show Altered Phosphate Starvation and Defense Responses
Transgenic Plants That Express the Phytoplasma Effector SAP11 Show Altered Phosphate Starvation and Defense Responses
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DOI：
10.1104/pp.113.229740
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发表时间：
2014-03-01
期刊：
影响因子：
7.4
通讯作者：
Yang, Jun-Yi
中科院分区：
文献类型：
--
作者：
Lu, Yen-Ting;Li, Meng-Ying;Yang, Jun-Yi
植原体具有细菌中最小的基因组，并且缺乏生物合成和代谢功能所需的许多必需基因，这使得它们成为不可培养的、韧皮部限制的植物病原体。在这项研究中，我们观察到表达分泌的 Aster Yellows 植原体菌株 Witches' Broom 蛋白 11 的转基因拟南芥 (Arabidopsis thaliana) 通过形成毛状根而显示出根结构的改变，类似于植原体感染植物的疾病症状。这种形态变化与细胞磷酸盐 (Pi) 的积累以及 Pi 饥饿诱导基因和 microRNA 表达水平的增加同时发生。除了 Pi 饥饿反应外，我们还发现分泌的 Aster Yellows 植原体菌株 Witches' Broom 蛋白 11 抑制水杨酸介导的防御反应并增强细菌病原体的生长。这些结果有助于更好地理解植原体效应子 SAP11 的作用，并为理解植物-病原体相互作用的分子基础提供新的见解。
Phytoplasmas have the smallest genome among bacteria and lack many essential genes required for biosynthetic and metabolic functions, making them unculturable, phloem-limited plant pathogens. In this study, we observed that transgenic Arabidopsis (Arabidopsis thaliana) expressing the secreted Aster Yellows phytoplasma strain Witches' Broom protein11 shows an altered root architecture, similarly to the disease symptoms of phytoplasma-infected plants, by forming hairy roots. This morphological change is paralleled by an accumulation of cellular phosphate (Pi) and an increase in the expression levels of Pi starvation-induced genes and microRNAs. In addition to the Pi starvation responses, we found that secreted Aster Yellows phytoplasma strain Witches' Broom protein11 suppresses salicylic acid-mediated defense responses and enhances the growth of a bacterial pathogen. These results contribute to an improved understanding of the role of phytoplasma effector SAP11 and provide new insights for understanding the molecular basis of plant-pathogen interactions.