Production and immobilization of lipase PCL and its application in synthesis of α-linolenic acid-rich diacylglycerol
Production and immobilization of lipase PCL and its application in synthesis of α-linolenic acid-rich diacylglycerol
复制标题
脂肪酶PCL的制备、固定化及其在富含α-亚麻酸二酰甘油合成中的应用
DOI：
10.1111/jfbc.12574
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发表时间：
2018-10-01
影响因子：
4
通讯作者：
Wang, Yonghua
中科院分区：
文献类型：
--
作者：
Liu, Nan;Li, Daoming;Wang, Yonghua
富含α-亚麻酸的二酰甘油已被证明具有良好的健康促进功能。本研究考察了脂肪酶PCL的生产、固定化及其在α-亚麻酸与甘油酯化合成二酰基甘油中的应用。选择树脂ECR8806作为脂肪酶PCL固定化的有效载体。傅里叶变换红外和激光扫描共聚焦显微镜分析证明脂肪酶成功固定在树脂上。与游离PCL相比，固定化PCL表现出更高的耐温性。在优化反应条件下，DAG含量达到54.49%。经过分子蒸馏进一步纯化后，DAG的纯度高达99.28%。酯化过程中，固定化的PCL非常稳定，10次循环后仍保留91.60%以上的初始活性。这些关于固定化PCL的新发现将使其成为油脂工业中的一种有前景的酶。固定化PCL是一种单酰基甘油和二酰基甘油脂肪酶，首先用于合成高纯度的富含α-亚麻酸的二酰基甘油。脂肪酶改进的生物催化性能和可重复使用性使其成为工业应用中有前途的催化剂。
alpha-Linolenic acid-rich diacylglycerol has been demonstrated with promising health promotion functions. This study examined the production, immobilization of lipase PCL, and its application in the synthesis of diacylglycerol by esterification of -linolenic acid with glycerol. The resin ECR8806 was selected as an effective support for the immobilization of lipase PCL. Fourier transform infrared and Laser scanning confocal microscope analysis proved that the lipase was successfully immobilized on the resin. Compared with the free PCL, the immobilized one exhibited higher temperature tolerance. Under optimized reaction conditions, a DAG content of 54.49% were obtained. After further purified by molecular distillation, the purity of DAG was up to 99.28%. During esterification, the immobilized PCL was quite stable and retained more than 91.60% of its initial activity after 10 cycles. These new findings on the immobilized PCL will make it to be a prospective enzyme in oils and fats industry.Immobilized PCL, a monoacylglycerol and diacylglycerol lipase, was first employed to synthesis of a high purity -linolenic acid-rich diacylglycerol. The improved biocatalytic performance and reusability of the lipase make it to be a promising catalyst in industrial application.