Long-term persistence of translocations in stable lymphocytes from victims of a radiological accident
Long-term persistence of translocations in stable lymphocytes from victims of a radiological accident
复制标题
DOI：
10.1080/09553000412331283498
复制
发表时间：
2004-08-01
影响因子：
2.6
通讯作者：
Edwards, A
中科院分区：
文献类型：
--
作者：
Lindholm, C;Edwards, A
目的：研究“稳定”淋巴细胞（即在任何染色体（包括复染染色体）中不含不稳定畸变的淋巴细胞）中的易位是否与在所有细胞中测量的易位相比具有更长的持续时间。材料和方法：对1994年11月爱沙尼亚一次事故中三名受照程度最高的辐射受害者的染色体畸变的时间进程进行了7年的跟踪，事故年份包括15个样本。使用1号、2号和4号染色体的探针和泛着丝粒探针进行染色体涂染，并使用畸变鉴定和命名术语（PAINT）命名法的开发版本对涉及涂染染色体的染色体畸变进行评分。用图像分析仪捕获含有畸变的中期，并储存在计算机上。在所有细胞中对基因组的着色部分中的畸变进行了早期分析，而不考虑基因组的未着色部分中可能的畸变。本分析考虑了完整细胞的“稳定/不稳定”性质。对图像进行评价，计数所有染色体，并检查复染染色体的不稳定畸变，即双着丝粒，无着丝粒或环形chromosome.Results：在所有细胞的原始分析，在早期样品中观察到易位频率下降。在目前的研究中，稳定的细胞，结果表明，产量的易位是恒定的时间。结论：结果表明，在稳定细胞中观察到的易位是持续的随着时间的推移。这意味着应使用稳定的电池进行回顾性剂量测定和校准。为了获得关于这个问题的更多信息，应注意在今后对辐射事故进行荧光原位杂交跟踪时所有细胞的稳定状态。
Purpose: To investigate whether translocations in 'stable' lymphocytes, i.e. those not containing unstable aberrations in any chromosome including counterstained ones, would have a longer persistence with time compared with those measured in all cells.Materials and methods: The time-course of chromosomal aberrations in the three most highly exposed radiation victims of an Estonian accident in November 1994 was followed for 7 post-accident years encompassing 15 samples. Chromosome painting was performed using probes for chromosomes 1, 2 and 4 with a pan-centromeric probe, and chromosomal aberrations involving the painted chromosomes were scored using a developed version of the Protocol for Aberration Identification and Nomenclature Terminology ( PAINT) nomenclature. Metaphases containing aberrations were captured with an image analyser and stored on a computer. An earlier analysis of aberrations in the painted portion of the genome was performed in all cells, irrespective of the possible aberrations in the unpainted part of the genome. The present analysis has taken into account the 'stable/unstable' nature of the complete cell. Evaluation was performed on images, counting all chromosomes and checking the counterstained chromosomes for unstable aberrations, i.e. dicentrics, acentrics or ring chromosomes.Results: In the original analyses of all cells, a decrease in translocation frequency in the early samples was observed. In the present study of stable cells, the results showed that the yield of translocations is constant with time. Conclusions: The results show that translocations observed in stable cells are persistent with time. This implies that retrospective dosimetry and calibration should be performed using stable cells. To obtain more information on this issue, the stability status of all cells in any future fluorescence in situ hybridization follow-up of a radiation accident should be noted.