Chondroitin sulfate is involved in the hypercalcification of the organic matrix of bovine peritubular dentin.
Chondroitin sulfate is involved in the hypercalcification of the organic matrix of bovine peritubular dentin.
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DOI：
10.1016/j.archoralbio.2015.11.008
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发表时间：
2016-02
影响因子：
3
通讯作者：
Veis A
中科院分区：
文献类型：
--
作者：
Dorvee JR;Gerkowicz L;Bahmanyar S;Deymier-Black A;Veis A
牙本质中的磷灰石矿物在成牙本质细胞突（OP）分泌的胶原基质（管间牙本质，ITD）中形成。高度钙化的矿物质（管周牙本质，PTD）沉积在ITD和每个过程膜之间的界面处，形成从牙本质-釉质接合处延伸到前牙本质-牙本质接合处的穿透牙本质的管状系统。我们专注于确定PTD的组成，无论是关于其有机基质和无机相。激光捕获技术已适用于隔离的矿化PTD的ITD，并通过SEM，TEM和能量色散光谱（EDS）的PTD的分析，并与类似的分析完整的牙本质切片ITD结合PTD环。元素线扫描清楚地标记了ITD，PTD和OP组件之间的边界，并揭示了组成和形貌表面粗糙度的差异。PTD的有机基质显示富含硫，进一步的抗体标记显示硫酸化的有机组分是硫酸软骨素B。在这种有机基质中的Ca/P比明显高于ITD和完整的PTD，表明多糖结合的S提供了阴离子平衡，促进了磷灰石PTD矿物的形成。
Apatitic mineral of dentin forms within the collagenous matrix (intertubular dentin, ITD) secreted from the odontoblastic processes (OP). Highly calcified mineral (peritubular dentin, PTD) is deposited at the interface between the ITD and each process membrane, creating a tubular system penetrating the dentin that extends from the dentino-enamel junction to the predentin-dentin junction. We focus on determining the composition of the PTD both with regard to its organic matrix and the inorganic phase. A laser capture technique has been adapted for isolation of the mineralized PTD free of the ITD, and for the analysis of the PTD by SEM, TEM, and energy dispersive spectrometry (EDS), and comparison with similar analyses of intact dentin slices containing ITD bounded-PTD annuli. Elemental line scans clearly marked the boundaries between ITD, PTD, and OP components, and revealed differences in composition, and topographical surface roughness. The organic matrix of the PTD was shown to be sulfur rich, and further antibody labeling showed the sulfated organic component to be chondroitin sulfate B. In this organic matrix the Ca/P ratio was distinctly higher than in the ITD and intact PTD, indicating that polysaccharide bound S supplies the anionic counterion facilitating the formation of the apatitic PTD mineral.