Microwave-induced inactivation of DNA-based hybrid catalyst in asymmetric catalysis.
Microwave-induced inactivation of DNA-based hybrid catalyst in asymmetric catalysis.
复制标题
不对称催化中基于 DNA 的混合催化剂的微波诱导失活。
DOI：
10.1016/j.ijbiomac.2015.12.048
复制
发表时间：
2016
影响因子：
8.2
通讯作者：
Shen,Kai
中科院分区：
文献类型：
--
作者：
Zhao,Hua;Shen,Kai
基于DNA的杂化催化剂在不对称反应中得到了广泛的关注。然而，为了保持较高的对映体选择性，这些反应通常在相对较低的温度(如<；5℃)下进行2-3天。为了提高反应的转化率，我们对微波辐射同时冷却作为潜在能源进行了评价，因为这种方法已经被广泛应用于各种化学反应和酶促反应。然而，我们的数据表明，即使在低温(如5℃)下，微波辐射也会导致DNA基杂化催化剂失活。圆二色谱(CD)和DNA凝胶电泳谱表明，微波辐射会降解DNA分子，破坏DNA双链结构，导致DNA与金属配体结合性质的改变，从而降低DNA的催化性能。
DNA-based hybrid catalysts have gained strong interests in asymmetric reactions. However, to maintain the high enantioselectivity, these reactions are usually conducted at relatively low temperatures (e.g. < 5 °C) for 2–3 days. Aiming to improve the reaction’s turnover rate, we evaluated microwave irradiation with simultaneous cooling as potential energy source since this method has been widely used to accelerate various chemical and enzymatic reactions. However, our data indicated that microwave irradiation induced an inactivation of DNA-based hybrid catalyst even at low temperatures (such as 5 °C). Circular dichroism (CD) spectra and gel electrophoresis of DNA suggest that microwave exposure degrades DNA molecules and disrupts DNA double-stranded structures, causing changes of DNA–metal ligand binding properties and thus poor DNA catalytic performance.