PLASMID RP4 AS A VECTOR REPLICON IN GENETIC ENGINEERING
PLASMID RP4 AS A VECTOR REPLICON IN GENETIC ENGINEERING
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DOI：
10.1038/255504a0
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发表时间：
1975-01-01
期刊：
影响因子：
64.8
通讯作者：
GRINTER, NJ
中科院分区：
文献类型：
--
作者：
JACOB, AE;GRINTER, NJ
目前，人们对构建含有来自各种细菌属的DNA片段的质粒非常感兴趣，目的是研究掺入的细菌DNA在不同细菌宿主中的遗传潜力的表达。我们报道，自传递质粒RP 4只有一个易被EcoRI酶切割的位点，因此应该是此类实验中非常合适的细菌DNA载体。
THERE is now considerable interest in the construction of plasmids containing DNA fragments from a variety of bacterial genera, for purposes of studying the expression of the genetic potentialities of the incorporated bacterial DNA in different bacterial hosts. We report that the self-transmissible plasmid RP4 has only one site susceptible to cutting by the enzymeEcoRI, and should therefore be a very suitable bacterial DNA vector in such experiments.