Chemical modification of β-lactoglobulin by quinones
Chemical modification of β-lactoglobulin by quinones
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DOI：
10.2298/jsc0305243n
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发表时间：
2003-01-01
影响因子：
1
通讯作者：
Sladic, D
中科院分区：
文献类型：
--
作者：
Novakovic, I;Vujcic, Z;Sladic, D
Avarone/avarol quinone/hydroquinone对。以及它们的衍生物显示出相当大的抗肿瘤活性。在这项工作中，共价修饰β-乳球蛋白。从牛奶中分离的avarone，其模型化合物2-叔丁基-1，4-苯醌。并研究了它们的几种烷硫基衍生物。采用紫外/维斯分光光度法、SDS聚丙烯酰胺凝胶电泳和等电聚焦等技术对修饰物进行了分析。SDS PAGE的结果表明发生了蛋白质的聚合。修饰后蛋白质的pI值向低值移动，表明赖氨酸氨基是β-乳球蛋白与醌类反应的主要位点。
The avarone/avarol quinone/hydroquinone couple. as well as their derivatives show considerable antitumor activity. In this work, covalent modifications of beta-lactogglobulin. isolated from cow milk by avarone, its model compound 2-tert-butyl-1,4-benzoquinone. and several of their alkylthio derivatives were studied. The techniques applied for as-saying the modifications were: UV/VIS spectrophotometry, SDS PAGE and isoelectrofocusing. The results of the SDS PAGE suggest that polymerisation of the protein occurs. The shift of the pI of the protein upon modification toward lower values indicates that lysine amino groups are the principal site of die reaction of beta-lactoglobulin with the quinones.