Hantavirus disease outbreak in Germany:: Limitations of routine serological diagnostics and clustering of virus sequences of human and rodent origin
Hantavirus disease outbreak in Germany:: Limitations of routine serological diagnostics and clustering of virus sequences of human and rodent origin
复制标题
DOI：
10.1128/jcm.02573-06
复制
发表时间：
2007-09-01
影响因子：
9.4
通讯作者：
Meisel, Helga
中科院分区：
文献类型：
--
作者：
Schilling, Stefan;Emmerich, Petra;Meisel, Helga
在欧洲，肾综合征出血热主要由感染Puumala病毒(PUUV)或Dobrava病毒引起。对德国东南部下巴伐利亚州一起汉坦病毒病暴发的31名患者，采用酶联免疫吸附试验、免疫荧光试验和免疫印迹分析进行了血清诊断。然而，在其中少数病例中，通过这些标准检测方法对抗体进行的PUUV特异性分型失败，需要在生物安全的3级条件下进行病毒中和检测，以验证该病毒类型的感染。用逆转录-聚合酶链式反应从3例患者的急性期血清中扩增出PUUV RNA，发现该RNA与捕获在同一地区的库鼠的病毒序列有很高的同源性。这些发现将疫情与在当地啮齿动物种群中流行的一种新的PULTV血统“巴伐利亚”联系在一起。与疫情有关的巴伐利亚州血统只与德国的其他PULTV血统有远亲关系。
In Europe, hemorrhagic fever with renal syndrome results mainly from infection with Puumala virus (PUUV) or Dobrava virus. For 31 patients from a hantavirus disease outbreak in Lower Bavaria, a district in southeast Germany, serodiagnosis was undertaken by enzyme-linked immuiniosorbent assay, immunofluorescence assay, and inummoblot analysis. In a few of these cases, however, PUUV-specific typing of antibodies by these standard assays failed and a virus neutralization assay under biosafet, level 3 conditions was required to verify the infection by this virus type. PUUV RNA was amplified by reverse transcription-PCR from acute-phase sera of three patients and was found to be very closely related to virus sequences obtained from bank voles (Clethrionomys glareolus) trapped in the same area. These findings link the outbreak with a novel PULTV lineage, "Bavaria," circulating in the local rodent population. The Bavaria lineage associated with the outbreak is only distantly related to other PULTV lineages from Germany.