A bacterial global regulator forms a prion.
A bacterial global regulator forms a prion.
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细菌的全局调节因子形成朊病毒。
DOI：
10.1126/science.aai7776
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Hochschild,Ann
中科院分区：
文献类型：
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作者：
Yuan,AndyH;Hochschild,Ann
朊病毒是一种自我繁殖的蛋白质聚集体，在真菌中作为基于蛋白质的遗传元素。虽然普遍存在于真核生物中，但尚未在细菌中发现朊病毒。我们发现一种细菌蛋白，肉毒杆菌的转录终止子Rho （Cb-Rho）可以形成朊病毒。我们确定了一个候选朊病毒形成结构域（cPrD） inCb-Rho，并表明它赋予cb - rho淀粉样变性，并且可以在功能上取代酵母朊病毒形成蛋白的PrD。此外，它的cPrD使cb - rho能够进入大肠杆菌的其他构象——一种有效终止转录的可溶性形式和一种功能受损的聚合、自我传播的朊病毒形式。朊病毒形式导致转录组的全基因组变化。因此，Cb-Rho在细菌中作为一种基于蛋白质的遗传元素起作用，这表明朊病毒的出现早于真核生物和细菌之间的进化分裂。
Prions are self-propagating protein aggregates that act as protein-based elements of inheritance in fungi. Although prevalent in eukaryotes, prions have not been identified in bacteria. Here we found that a bacterial protein, transcription terminator Rho ofClostridium botulinum(Cb-Rho), could form a prion. We identified a candidate prion-forming domain (cPrD) inCb-Rho and showed that it conferred amyloidogenicity onCb-Rho and could functionally replace the PrD of a yeast prion-forming protein. Furthermore, its cPrD enabledCb-Rho to access alternative conformations inEscherichia coli—a soluble form that terminated transcription efficiently and an aggregated, self-propagating prion form that was functionally compromised. The prion form caused genome-wide changes in the transcriptome. Thus,Cb-Rho functions as a protein-based element of inheritance in bacteria, suggesting that the emergence of prions predates the evolutionary split between eukaryotes and bacteria.