Expression of Frizzleds and secreted frizzled-related proteins (Sfrps) during mammalian lens development
Expression of Frizzleds and secreted frizzled-related proteins (Sfrps) during mammalian lens development
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DOI：
10.1387/ijdb.041882yc
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发表时间：
2004-01-01
影响因子：
0.7
通讯作者：
McAvoy, JW
中科院分区：
文献类型：
--
作者：
Chen, YJ;Stump, RJW;McAvoy, JW
最近的研究表明Wnt信号传导在调节透镜细胞分化中的作用（Stump等人，2003年）。在这里，我们调查的表达模式的Wnt受体，卷曲蛋白（Fzs）和Wnt信号调节，分泌卷曲相关蛋白（Sfrps），在啮齿动物透镜的发展。RT-PCR结果显示Fz受体Fz 1-Fz 8在透镜中均有表达。原位杂交结果表明，所有的Fz基因检测具有相似的表达模式。Fz在整个早期透镜原基中表达。在胚胎第14.5天（E14.5），Fz基因表达主要定位于透镜赤道部的上皮和伸长细胞。透镜纤维中无Fz表达。这种Fz基因表达模式在整个出生后早期发育过程中持续存在。免疫定位研究表明，Fz蛋白的分布密切遵循的mRNA。此外，FGF处理的外植体中的上皮细胞在它们迁移和伸长时在细胞突起中显示最强的Fz反应性。Sfrp 1-Sfrp 5表达，并且除了Sfrp 2之外，在透镜发育期间，它们彼此之间以及与Fz具有相似的表达模式。Sfrp 2在所有透镜小凹细胞中强烈表达，但变得限于透镜囊泡的假定上皮细胞。到E14.5，Sfrp 2仅存在于透镜赤道上方的少数细胞中。在E18.5或之后的阶段，在透镜中未检测到Sfrp 2。本研究表明，多个Fz和Sfrp基因表达在透镜形态发生和分化。这与Wnt-Fz信号在胚胎和出生后透镜发育过程中的作用一致。
Recent studies indicate a role for Wnt signaling in regulating lens cell differentiation (Stump et al., 2003). Here we investigated expression patterns of Wnt receptors, the Frizzleds (Fzs) and the Wnt signaling regulators, the secreted frizzled-related proteins (Sfrps), during rodent lens development. RT-PCR showed that Fz receptors, Fz1-Fz8 are expressed in lens. In situ hybridization showed that all the Fz genes examined have similar expression patterns. Fzs are expressed throughout the early lens primordium. At embryonic day 14.5 (E14.5), Fz gene expression is predominantly localized to the epithelium and elongating cells at the lens equator. Fz expression is absent from lens fibers. This pattern of Fz gene expression continues throughout early postnatal development. Immunolocalization studies showed that Fz protein distribution closely follows that of the mRNAs. In addition, epithelial cells in FGF-treated explants show strongest Fz reactivity in cellular protrusions as they migrate and elongate. Sfrp1-Sfrp5 are expressed and all, except Sfrp2, have similar patterns of expression to each other and to the Fzs during lens development. Sfrp2 is strongly expressed in all lens pit cells but becomes restricted to the presumptive epithelial cells of the lens vesicle. By E14.5, Sfrp2 is only present in a few cells above the lens equator. Sfrp2 is not detected in the lens at E18.5 or at later stages. This study shows that multiple Fz and Sfrp genes are expressed during lens morphogenesis and differentiation. This is consistent with a role for Wnt-Fz signaling during both embryonic and postnatal lens development.