Cholera toxin B conjugated quantum dots for live cell Labeling
Cholera toxin B conjugated quantum dots for live cell Labeling
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DOI：
10.1021/nl0709930
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发表时间：
2007-09-01
期刊：
影响因子：
10.8
通讯作者：
Ballou, Byron
中科院分区：
文献类型：
--
作者：
Chakraborty, Subhasish K.;Fitzpatrick, James A. J.;Ballou, Byron
霍乱毒素亚单位B（CT B）-量子点偶联物可用于标记哺乳动物细胞。缀合物被所有测试的细胞系内化成分散在整个细胞质中的小囊泡，而市售的聚精氨酸缀合物迅速积累在大的核周内体中。尽管大比例的CTB缀合物最终也在核周内体中积累，但这种积累需要数天，并且即使如此，许多CTB缀合的量子点仍保持在分散在整个细胞质中的小囊泡中。因此，CTB缀合物是用于哺乳动物细胞的一般标记的聚精氨酸缀合物的实用替代物。
Cholera toxin subunit B (CTB)-quantum dot conjugates were developed for labeling mammalian cells. The conjugates were internalized by all tested cell lines into small vesicles dispersed throughout the cytoplasm, while commercially available polyarginine conjugates rapidly accumulated in large perinuclear endosomes. Although a large proportion of CTB conjugates eventually also accumulated in perinuclear endosomes, this accumulation required several days, and even then many CTB conjugated quantum dots remained in small vesicles dispersed throughout the cytoplasm. Thus CTB conjugates are a practical alternative to polyarginine conjugates for the general labeling of mammalian cells.